PBMC-derived T cells

Daniel Lozano-Ojalvo; Rosina López-Fandiño; Iván López-Expósito

doi:10.1007/978-3-319-16104-4_16

PBMC-derived T cells

Daniel Lozano-Ojalvo, Rosina López-Fandiño, Iván López-Expósito

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

13 Scopus citations

Abstract

T cell cultures are a valuable tool in food research to perform studies within the food allergy field. Their main applications aim to analyze immunological responses towards food protein antigens to gain further insights into the mechanisms responsible for the development of oral tolerance or for the triggering of food allergies. This chapter describes the main applications, isolation techniques, and culture conditions for PBMC-derived T cells. Furthermore, critical parameters of the model, together with the experimental read outs will be discussed.

Original language	English
Title of host publication	The Impact of Food Bioactives on Health
Subtitle of host publication	In Vitro and Ex Vivo Models
Publisher	Springer International Publishing
Pages	169-180
Number of pages	12
ISBN (Electronic)	9783319161044
ISBN (Print)	9783319157917
DOIs	https://doi.org/10.1007/978-3-319-16104-4_16
State	Published - 1 Jan 2015
Externally published	Yes

Keywords

CD4T cells
Food allergy
Immunomagnetic cell isolation
PBMCs
T cells cultures

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10.1007/978-3-319-16104-4_16

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abstract = "T cell cultures are a valuable tool in food research to perform studies within the food allergy field. Their main applications aim to analyze immunological responses towards food protein antigens to gain further insights into the mechanisms responsible for the development of oral tolerance or for the triggering of food allergies. This chapter describes the main applications, isolation techniques, and culture conditions for PBMC-derived T cells. Furthermore, critical parameters of the model, together with the experimental read outs will be discussed.",

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AU - Lozano-Ojalvo, Daniel

AU - López-Fandiño, Rosina

AU - López-Expósito, Iván

N1 - Publisher Copyright: © The Editor(s) (if applicable) and the Author(s) 2015.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - T cell cultures are a valuable tool in food research to perform studies within the food allergy field. Their main applications aim to analyze immunological responses towards food protein antigens to gain further insights into the mechanisms responsible for the development of oral tolerance or for the triggering of food allergies. This chapter describes the main applications, isolation techniques, and culture conditions for PBMC-derived T cells. Furthermore, critical parameters of the model, together with the experimental read outs will be discussed.

AB - T cell cultures are a valuable tool in food research to perform studies within the food allergy field. Their main applications aim to analyze immunological responses towards food protein antigens to gain further insights into the mechanisms responsible for the development of oral tolerance or for the triggering of food allergies. This chapter describes the main applications, isolation techniques, and culture conditions for PBMC-derived T cells. Furthermore, critical parameters of the model, together with the experimental read outs will be discussed.

KW - CD4T cells

KW - Food allergy

KW - Immunomagnetic cell isolation

KW - PBMCs

KW - T cells cultures

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SP - 169

EP - 180

BT - The Impact of Food Bioactives on Health

PB - Springer International Publishing

ER -

PBMC-derived T cells

Abstract

Keywords

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