Patterns of retinal ganglion cell survival after brain-derived neurotrophic factor administration in hypertensive eyes of rats

Mei Lan Ko; Dan Ning Hu; Robert Ritch; S. C. Sharma; Chau Fong Chen

doi:10.1016/S0304-3940(01)01830-4

Patterns of retinal ganglion cell survival after brain-derived neurotrophic factor administration in hypertensive eyes of rats

Mei Lan Ko
, Dan Ning Hu
, Robert Ritch
, S. C. Sharma
, Chau Fong Chen

Research output: Contribution to journal › Article › peer-review

146 Scopus citations

Abstract

We investigated the effect of brain-derived neurotrophic factor (BDNF) on retinal ganglion cell (RGC) survival after intraocular pressure (IOP) elevation at various time intervals. In adult Wistar rats, RGCs were labeled with 5% Fluorogold. Animals with 1.8-2.5-fold increase in IOP after cauterization of three episcleral vessels, were divided into three BDNF groups and three vehicle control groups, each receiving one, two or three injections. The RGC survival percentage on RGCs of the first, second and third injections were 93.9% (n = 7), 91.3% (n = 7), 82.7% (n = 5), respectively in BDNF groups; 91.6% (n = 6), 84.1% (n = 6) and 73.5% (n = 5), respectively in vehicle controls. The second and third injections of BDNF showed statistically significant survival effects. These findings demonstrated that BDNF has partial neuroprotection on RGCs in whole retina and enhances RGC survival in moderately chronic hypertensive eyes.

Original language	English
Pages (from-to)	139-142
Number of pages	4
Journal	Neuroscience Letters
Volume	305
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(01)01830-4
State	Published - 8 Jun 2001
Externally published	Yes

Keywords

Brain-derived neurotrophic factor
Glaucoma
Hypertensive eye
Retinal ganglion cell

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10.1016/S0304-3940(01)01830-4

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@article{b825620f7ef44e159f2246abefcf5aee,

title = "Patterns of retinal ganglion cell survival after brain-derived neurotrophic factor administration in hypertensive eyes of rats",

abstract = "We investigated the effect of brain-derived neurotrophic factor (BDNF) on retinal ganglion cell (RGC) survival after intraocular pressure (IOP) elevation at various time intervals. In adult Wistar rats, RGCs were labeled with 5\% Fluorogold. Animals with 1.8-2.5-fold increase in IOP after cauterization of three episcleral vessels, were divided into three BDNF groups and three vehicle control groups, each receiving one, two or three injections. The RGC survival percentage on RGCs of the first, second and third injections were 93.9\% (n = 7), 91.3\% (n = 7), 82.7\% (n = 5), respectively in BDNF groups; 91.6\% (n = 6), 84.1\% (n = 6) and 73.5\% (n = 5), respectively in vehicle controls. The second and third injections of BDNF showed statistically significant survival effects. These findings demonstrated that BDNF has partial neuroprotection on RGCs in whole retina and enhances RGC survival in moderately chronic hypertensive eyes.",

keywords = "Brain-derived neurotrophic factor, Glaucoma, Hypertensive eye, Retinal ganglion cell",

author = "Ko, \{Mei Lan\} and Hu, \{Dan Ning\} and Robert Ritch and Sharma, \{S. C.\} and Chen, \{Chau Fong\}",

note = "Funding Information: We are grateful to Mr. Ming-Chieh Ma and Miss Terry Liu for helping the experiment and to Dr. Dar-Shaong Hwang for biostatistics. Supported by Department of Ophthalmology Research Fund, NYEE, Glaucoma Foundation, New York, NY; Leon Lowenstein Foundation, Achelis and Bodman Foundation, and NIH, EY 11295, NSC 89–2320-B002–171.",

year = "2001",

month = jun,

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doi = "10.1016/S0304-3940(01)01830-4",

language = "English",

volume = "305",

pages = "139--142",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "2",

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TY - JOUR

T1 - Patterns of retinal ganglion cell survival after brain-derived neurotrophic factor administration in hypertensive eyes of rats

AU - Ko, Mei Lan

AU - Hu, Dan Ning

AU - Ritch, Robert

AU - Sharma, S. C.

AU - Chen, Chau Fong

N1 - Funding Information: We are grateful to Mr. Ming-Chieh Ma and Miss Terry Liu for helping the experiment and to Dr. Dar-Shaong Hwang for biostatistics. Supported by Department of Ophthalmology Research Fund, NYEE, Glaucoma Foundation, New York, NY; Leon Lowenstein Foundation, Achelis and Bodman Foundation, and NIH, EY 11295, NSC 89–2320-B002–171.

PY - 2001/6/8

Y1 - 2001/6/8

N2 - We investigated the effect of brain-derived neurotrophic factor (BDNF) on retinal ganglion cell (RGC) survival after intraocular pressure (IOP) elevation at various time intervals. In adult Wistar rats, RGCs were labeled with 5% Fluorogold. Animals with 1.8-2.5-fold increase in IOP after cauterization of three episcleral vessels, were divided into three BDNF groups and three vehicle control groups, each receiving one, two or three injections. The RGC survival percentage on RGCs of the first, second and third injections were 93.9% (n = 7), 91.3% (n = 7), 82.7% (n = 5), respectively in BDNF groups; 91.6% (n = 6), 84.1% (n = 6) and 73.5% (n = 5), respectively in vehicle controls. The second and third injections of BDNF showed statistically significant survival effects. These findings demonstrated that BDNF has partial neuroprotection on RGCs in whole retina and enhances RGC survival in moderately chronic hypertensive eyes.

AB - We investigated the effect of brain-derived neurotrophic factor (BDNF) on retinal ganglion cell (RGC) survival after intraocular pressure (IOP) elevation at various time intervals. In adult Wistar rats, RGCs were labeled with 5% Fluorogold. Animals with 1.8-2.5-fold increase in IOP after cauterization of three episcleral vessels, were divided into three BDNF groups and three vehicle control groups, each receiving one, two or three injections. The RGC survival percentage on RGCs of the first, second and third injections were 93.9% (n = 7), 91.3% (n = 7), 82.7% (n = 5), respectively in BDNF groups; 91.6% (n = 6), 84.1% (n = 6) and 73.5% (n = 5), respectively in vehicle controls. The second and third injections of BDNF showed statistically significant survival effects. These findings demonstrated that BDNF has partial neuroprotection on RGCs in whole retina and enhances RGC survival in moderately chronic hypertensive eyes.

KW - Brain-derived neurotrophic factor

KW - Glaucoma

KW - Hypertensive eye

KW - Retinal ganglion cell

UR - https://www.scopus.com/pages/publications/0035827703

U2 - 10.1016/S0304-3940(01)01830-4

DO - 10.1016/S0304-3940(01)01830-4

M3 - Article

C2 - 11376903

AN - SCOPUS:0035827703

SN - 0304-3940

VL - 305

SP - 139

EP - 142

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2

ER -

Patterns of retinal ganglion cell survival after brain-derived neurotrophic factor administration in hypertensive eyes of rats

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Keywords

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