@inproceedings{0fe4d31672de407db8e9cf93693181a5,
title = "Patterning micro-stiffness in cell-adhesive substrate using microfluidics-based lithography",
abstract = "In this study, we demonstrate the formation of 3D cell-adhesive hydrogels exhibiting well-defined spatial variation in stiffness using our previously developed microfluidics-based lithography technique [12]. PEG monoacrylate-linked bovine fibrinogen (PEG-fibrinogen) is photopolymerized into specific, user-defined shapes inside a microchannel, and successive cycles of fabrication result in a heterogeneous structure with controlled regional variations in stiffness. Atomic force microscope (AFM) indentations were used to directly confirm the micro-stiffness distribution, and morphological and migratory behavior of cells in microenvironments of controlled variations in stiffness was characterized. Our approach allows control of microscale variations of stiffness in cell-adhesive substrates with high precision and flexibility and offers the opportunity to examine differential cell-ECM interactions relevant to a multitude of fundamental cellular processes [14].",
author = "Cheung, \{Yuk Kee\} and Azeloglu, \{Evren U.\} and David Shiovitz and Costa, \{Kevin D.\} and Dror Seliktar and Sia, \{Samuel K.\}",
year = "2010",
doi = "10.1109/NEBC.2010.5458111",
language = "English",
isbn = "9781424468799",
series = "Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010",
booktitle = "Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010",
note = "36th Annual Northeast Bioengineering Conference, NEBEC 2010 ; Conference date: 26-03-2010 Through 28-03-2010",
}