TY - GEN
T1 - Patterning micro-stiffness in cell-adhesive substrate using microfluidics-based lithography
AU - Cheung, Yuk Kee
AU - Azeloglu, Evren U.
AU - Shiovitz, David
AU - Costa, Kevin D.
AU - Seliktar, Dror
AU - Sia, Samuel K.
PY - 2010
Y1 - 2010
N2 - In this study, we demonstrate the formation of 3D cell-adhesive hydrogels exhibiting well-defined spatial variation in stiffness using our previously developed microfluidics-based lithography technique [12]. PEG monoacrylate-linked bovine fibrinogen (PEG-fibrinogen) is photopolymerized into specific, user-defined shapes inside a microchannel, and successive cycles of fabrication result in a heterogeneous structure with controlled regional variations in stiffness. Atomic force microscope (AFM) indentations were used to directly confirm the micro-stiffness distribution, and morphological and migratory behavior of cells in microenvironments of controlled variations in stiffness was characterized. Our approach allows control of microscale variations of stiffness in cell-adhesive substrates with high precision and flexibility and offers the opportunity to examine differential cell-ECM interactions relevant to a multitude of fundamental cellular processes [14].
AB - In this study, we demonstrate the formation of 3D cell-adhesive hydrogels exhibiting well-defined spatial variation in stiffness using our previously developed microfluidics-based lithography technique [12]. PEG monoacrylate-linked bovine fibrinogen (PEG-fibrinogen) is photopolymerized into specific, user-defined shapes inside a microchannel, and successive cycles of fabrication result in a heterogeneous structure with controlled regional variations in stiffness. Atomic force microscope (AFM) indentations were used to directly confirm the micro-stiffness distribution, and morphological and migratory behavior of cells in microenvironments of controlled variations in stiffness was characterized. Our approach allows control of microscale variations of stiffness in cell-adhesive substrates with high precision and flexibility and offers the opportunity to examine differential cell-ECM interactions relevant to a multitude of fundamental cellular processes [14].
UR - http://www.scopus.com/inward/record.url?scp=77953048044&partnerID=8YFLogxK
U2 - 10.1109/NEBC.2010.5458111
DO - 10.1109/NEBC.2010.5458111
M3 - Conference contribution
AN - SCOPUS:77953048044
SN - 9781424468799
T3 - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
BT - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
T2 - 36th Annual Northeast Bioengineering Conference, NEBEC 2010
Y2 - 26 March 2010 through 28 March 2010
ER -