Partial Purification and Characterisation of a Renal Growth Factor from Plasma of Uninephrectomised Rats

F. Manzano; P. Esbrit; A. Garcia-Ocana; R. Garcia-Canero; M. A. Jimenez-Clavero

doi:10.1093/oxfordjournals.ndt.a091886

Partial Purification and Characterisation of a Renal Growth Factor from Plasma of Uninephrectomised Rats

F. Manzano, P. Esbrit, A. Garcia-Ocana, R. Garcia-Canero, M. A. Jimenez-Clavero

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7 Scopus citations

Abstract

Renal growth factor activity was extracted from plasma of adult uninephrectomised rats and partially purified by gel filtration and anion-exchanger FPLC. It induced a maximal stimulation of mouse DNA synthesis in vivo at 1.75 ug/mouse. In addition, renal growth factor was found to maximally stimulate DNA synthesis in LLC-PKj cells at 150 ng/ml. This maximal response was then found to decrease with higher doses of renal growth factor, in vivo and in vitro. The apparent molecular weight of renal growth factor was estimated to be 17 K-22 K by gel filtration. It was found to be resistant to heat and to trypsin, but labile to reduction with dithiothreitol.

Original language	English
Pages (from-to)	334-338
Number of pages	5
Journal	Nephrology Dialysis Transplantation
Volume	4
Issue number	5
DOIs	https://doi.org/10.1093/oxfordjournals.ndt.a091886
State	Published - 1989
Externally published	Yes

Keywords

Cells
DNA synthesis
LLC-PK
Renal growth factor
Uninephrectomy

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10.1093/oxfordjournals.ndt.a091886

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title = "Partial Purification and Characterisation of a Renal Growth Factor from Plasma of Uninephrectomised Rats",

abstract = "Renal growth factor activity was extracted from plasma of adult uninephrectomised rats and partially purified by gel filtration and anion-exchanger FPLC. It induced a maximal stimulation of mouse DNA synthesis in vivo at 1.75 ug/mouse. In addition, renal growth factor was found to maximally stimulate DNA synthesis in LLC-PKj cells at 150 ng/ml. This maximal response was then found to decrease with higher doses of renal growth factor, in vivo and in vitro. The apparent molecular weight of renal growth factor was estimated to be 17 K-22 K by gel filtration. It was found to be resistant to heat and to trypsin, but labile to reduction with dithiothreitol.",

keywords = "Cells, DNA synthesis, LLC-PK, Renal growth factor, Uninephrectomy",

author = "F. Manzano and P. Esbrit and A. Garcia-Ocana and R. Garcia-Canero and Jimenez-Clavero, {M. A.}",

note = "Funding Information: Acknowledgements. We are grateful to Dr J. J. Diaz-Gil for helpful comments. We thank Dr C. Castilla for skilled surgical assistance. We also thank Liselotte Gulliksen for typing the manuscript. This work was partially supported by a grant from the National Institute of Health of Spain. F.M. is a fellow of Conchita Rabago Foundation.",

year = "1989",

doi = "10.1093/oxfordjournals.ndt.a091886",

language = "English",

volume = "4",

pages = "334--338",

journal = "Nephrology Dialysis Transplantation",

issn = "0931-0509",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Partial Purification and Characterisation of a Renal Growth Factor from Plasma of Uninephrectomised Rats

AU - Manzano, F.

AU - Esbrit, P.

AU - Garcia-Ocana, A.

AU - Garcia-Canero, R.

AU - Jimenez-Clavero, M. A.

N1 - Funding Information: Acknowledgements. We are grateful to Dr J. J. Diaz-Gil for helpful comments. We thank Dr C. Castilla for skilled surgical assistance. We also thank Liselotte Gulliksen for typing the manuscript. This work was partially supported by a grant from the National Institute of Health of Spain. F.M. is a fellow of Conchita Rabago Foundation.

PY - 1989

Y1 - 1989

N2 - Renal growth factor activity was extracted from plasma of adult uninephrectomised rats and partially purified by gel filtration and anion-exchanger FPLC. It induced a maximal stimulation of mouse DNA synthesis in vivo at 1.75 ug/mouse. In addition, renal growth factor was found to maximally stimulate DNA synthesis in LLC-PKj cells at 150 ng/ml. This maximal response was then found to decrease with higher doses of renal growth factor, in vivo and in vitro. The apparent molecular weight of renal growth factor was estimated to be 17 K-22 K by gel filtration. It was found to be resistant to heat and to trypsin, but labile to reduction with dithiothreitol.

AB - Renal growth factor activity was extracted from plasma of adult uninephrectomised rats and partially purified by gel filtration and anion-exchanger FPLC. It induced a maximal stimulation of mouse DNA synthesis in vivo at 1.75 ug/mouse. In addition, renal growth factor was found to maximally stimulate DNA synthesis in LLC-PKj cells at 150 ng/ml. This maximal response was then found to decrease with higher doses of renal growth factor, in vivo and in vitro. The apparent molecular weight of renal growth factor was estimated to be 17 K-22 K by gel filtration. It was found to be resistant to heat and to trypsin, but labile to reduction with dithiothreitol.

KW - Cells

KW - DNA synthesis

KW - LLC-PK

KW - Renal growth factor

KW - Uninephrectomy

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DO - 10.1093/oxfordjournals.ndt.a091886

M3 - Article

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AN - SCOPUS:0024321619

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SP - 334

EP - 338

JO - Nephrology Dialysis Transplantation

JF - Nephrology Dialysis Transplantation

SN - 0931-0509

IS - 5

ER -

Partial Purification and Characterisation of a Renal Growth Factor from Plasma of Uninephrectomised Rats

Abstract

Keywords

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