TY - JOUR
T1 - Partial depolymerization of pectin by a photochemical reaction
AU - Burana-osot, Jankana
AU - Soonthornchareonnon, Noppamas
AU - Hosoyama, Saori
AU - Linhardt, Robert J.
AU - Toida, Toshihiko
N1 - Funding Information:
This work was supported in part by the Japan Society for the Promotion of Science (JSPS)—Ronpaku (Dissertation PhD) Program.
PY - 2010/6/16
Y1 - 2010/6/16
N2 - Complex heterogeneous polysaccharides that comprise pectin were partially depolymerized by a photochemical reaction using ultraviolet light in the presence of titanium dioxide catalyst. In a period of 6 h at pH 7, this UV/TiO2 process decreased the average molecular weight of pectin from 400 kDa to 200 kDa. The characterization of the partially depolymerized pectin, which was fractionated by size-exclusion chromatography, was performed by 1H NMR spectroscopy, and the spectra obtained showed that the resulting oligosaccharides and polysaccharides maintained the intact core structure of pectin. The monosaccharide content and depolymerization profile were determined by high-performance anion-exchange chromatography coupled with pulsed amperometric detection. This controlled photochemical depolymerization technique might be useful for preparation of pectin oligosaccharides as an ingredient in food and pharmaceutical products.
AB - Complex heterogeneous polysaccharides that comprise pectin were partially depolymerized by a photochemical reaction using ultraviolet light in the presence of titanium dioxide catalyst. In a period of 6 h at pH 7, this UV/TiO2 process decreased the average molecular weight of pectin from 400 kDa to 200 kDa. The characterization of the partially depolymerized pectin, which was fractionated by size-exclusion chromatography, was performed by 1H NMR spectroscopy, and the spectra obtained showed that the resulting oligosaccharides and polysaccharides maintained the intact core structure of pectin. The monosaccharide content and depolymerization profile were determined by high-performance anion-exchange chromatography coupled with pulsed amperometric detection. This controlled photochemical depolymerization technique might be useful for preparation of pectin oligosaccharides as an ingredient in food and pharmaceutical products.
KW - HPAEC-PAD
KW - NMR spectroscopy
KW - Pectin
KW - Photochemical depolymerization
UR - http://www.scopus.com/inward/record.url?scp=77953131696&partnerID=8YFLogxK
U2 - 10.1016/j.carres.2010.04.007
DO - 10.1016/j.carres.2010.04.007
M3 - Article
C2 - 20451177
AN - SCOPUS:77953131696
SN - 0008-6215
VL - 345
SP - 1205
EP - 1210
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 9
ER -