Abstract
Gene targeting by homologous recombination provides a precise mechanism for defined genomic modifications in embryonic stem cells. This is important for studying gene function in vitro, as well as for lineage selection and marking. The following provides the details of this technique and a strategy for genetically manipulating human ES cells by homologous recombination. [Note for author- What is the typical frequency of targeting? What are the principle variables affecting this frequency?]
| Original language | English |
|---|---|
| Title of host publication | Human Embryonic Stem Cells |
| Subtitle of host publication | The Practical Handbook |
| Publisher | John Wiley & Sons, Ltd. |
| Pages | 357-365 |
| Number of pages | 9 |
| ISBN (Print) | 9780470033562 |
| DOIs | |
| State | Published - 15 Jun 2007 |
| Externally published | Yes |
Keywords
- Electroporation
- Gene targeting
- Homologous recombination
- Human embryonic stem cell
- Knock in
- Knock out