Parathyroid hormone-related protein (107-139) decreases alkaline phosphatase in osteoblastic osteosarcoma cells UMR 106 by a protein kinase C- dependent pathway

A. Valín, F. De Miguel, A. García-Ocaña, P. Esbrit

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11 Scopus citations

Abstract

The C-terminal (107-111) region of parathyroid hormone-related protein (PTHrP) appears to inhibit osteoclastic bone resorption, and to affect osteoblastic growth and differentiation. We tested the effect of human PTHrP (107-139) on alkaline phosphatase (ALP) activity in osteoblastic osteosarcoma UMR 106 cells. We found that this C-terminal PTHrP peptide, between 10 nM and 10 fM, inhibited ALP activity in these cells during the log phase of growth. Human PTHrP (1-34) amide and human PTHrP (1-141) were as potent as PTHrP (107-139) in growing UMR 106 cells. This inhibitory effect of 10 nM PTHrP (107-139) on ALP activity was also observed in serum-depleted cells, and in the presence of 10 nM dexamethasone, which increased ALP activity by 40% in these cells. In addition, this effect of PTHrP (107-139) was blunted by 25 nM bisindolylmaleimide I, a protein kinase C inhibitor. These results support a role for the C-terminal region of PTHrP as a modulator of bone formation.

Original languageEnglish
Pages (from-to)148-151
Number of pages4
JournalCalcified Tissue International
Volume65
Issue number2
DOIs
StatePublished - 1999
Externally publishedYes

Keywords

  • Alkaline phosphatase
  • C-terminal
  • Osteoblastic-like cells
  • Parathyroid hormone-related protein
  • Protein kinase C
  • UMR 106 cells

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