Paramagnetic lipid-coated silica nanoparticles with a fluorescent quantum dot core: A new contrast agent platform for multimodality imaging

Rolf Koole, Matti M. Van Schooneveld, Jan Hilhorst, Karolien Castermans, David P. Cormode, Gustav J. Strijkers, Celso De Mello Donegá, Daniel Vanmaekelbergh, Arjan W. Griffioen, Klaas Nicolay, Zahi A. Fayad, Andries Meijerink, Willem J.M. Mulder

Research output: Contribution to journalArticlepeer-review

143 Scopus citations

Abstract

Silica particles as a nanoparticulate carrier material for contrast agents have received considerable attention the past few years, since the material holds great promise for biomedical applications. A key feature for successful application of this material in vivo is biocompatibility, which may be significantly improved by appropriate surface modification. In this study, we report a novel strategy to coat silica particles with a dense monolayer of paramagnetic and PEGylated lipids. The silica nanoparticles carry a quantum dot in their center and are made target-specific by the conjugation of multiple αvβ3-integrin-specific RGD-peptides. We demonstrate their specific uptake by endothelial cells in vitro using fluorescence microscopy, quantitative fluorescence imaging, and magnetic resonance imaging. The lipid-coated silica particles introduced here represent a new platform for nanoparticulate multimodality contrast agents.

Original languageEnglish
Pages (from-to)2471-2479
Number of pages9
JournalBioconjugate Chemistry
Volume19
Issue number12
DOIs
StatePublished - 17 Dec 2008

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