@article{e966c5e7426340b38697d537caacaf81,
title = "PAR-1 Phosphorylates Mind Bomb to Promote Vertebrate Neurogenesis",
abstract = "Generation of neurons in the vertebrate central nervous system requires a complex transcriptional regulatory network and signaling processes in polarized neuroepithelial progenitor cells. Here we demonstrate that neurogenesis in the Xenopus neural plate in vivo and mammalian neural progenitors in vitro involves intrinsic antagonistic activities of the polarity proteins PAR-1 and aPKC. Furthermore, we show that Mind bomb (Mib), a ubiquitin ligase that promotes Notch ligand trafficking and activity, is a crucial molecular substrate for PAR-1. The phosphorylation of Mib by PAR-1 results in Mib degradation, repression of Notch signaling, and stimulation of neuronal differentiation. These observations suggest a conserved mechanism for neuronal fate determination that might operate during asymmetric divisions of polarized neural progenitor cells.",
keywords = "CELLBIO, DEVBIO",
author = "Olga Ossipova and Jerome Ezan and Sokol, {Sergei Y.}",
note = "Funding Information: We thank A. Chitnis, E. Fuchs, P. Gergen, V. Joukov, C. Kintner, J. Kitajewski, P. Krieg, Q. Ma, Z. Pan, G. Thomsen, and G. Weinmaster for plasmids, E. Snyder for C17.2 cells, L. Gusella and A. Terskikh for help with lentiviral preparations, M. Klymkowsky for the Sox3 antibody, R. Cagan, M. Rendl, and A. Sproul for reading the manuscript, and D. Weinstein, T. Haremaki, and members of the Sokol laboratory for discussions. We especially thank J. Green for his support and discussions at the early stages of this project. Initial discovery of the effect of PAR-1 on neuronal differentiation was first made by O.O. in the Green laboratory. This study is devoted to the memory of Joseph L. Chertkov, an outstanding scientist and mentor. This work was supported by the NIH (S.S.) and the Grant Lavoisier and the Philippe Foundation fellowship (J.E.). ",
year = "2009",
month = aug,
day = "18",
doi = "10.1016/j.devcel.2009.06.010",
language = "English",
volume = "17",
pages = "222--233",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "2",
}