Palm-Sized Lab-In-A-Magnetofluidic Tube Platform for Rapid and Sensitive Virus Detection

Ziyue Li, Shuo Zhang, Jiongyu Zhang, Lori Avery, David Banach, Hui Zhao, Changchun Liu

Research output: Contribution to journalArticlepeer-review

Abstract

Simple, sensitive, and accurate molecular diagnostics are critical for preventing rapid spread of infection and initiating early treatment of diseases. However, current molecular detection methods typically rely on extensive nucleic acid sample preparation and expensive instrumentation. Here, a simple, fully integrated, lab-in-a-magnetofluidic tube (LIAMT) platform is presented for “sample-to-result” molecular detection of virus. By leveraging magnetofluidic transport of micro/nano magnetic beads, the LIAMT device integrates viral lysis, nucleic acid extraction, isothermal amplification, and CRISPR detection within a single engineered microcentrifuge tube. To enable point-of-care molecular diagnostics, a palm-sized processor is developed for magnetofluidic separation, nucleic acid amplification, and visual fluorescence detection. The LIAMT platform is applied to detect SARS-CoV-2 and HIV viruses, achieving a detection sensitivity of 73.4 and 63.9 copies µL−1, respectively. Its clinical utility is further demonstrated by detecting SARS-CoV-2 and HIV in clinical samples. This simple, affordable, and portable LIAMT platform holds promise for rapid and sensitive molecular diagnostics of infectious diseases at the point-of-care.

Original languageEnglish
Article number2310066
JournalAdvanced Science
Volume11
Issue number23
DOIs
StatePublished - 19 Jun 2024
Externally publishedYes

Keywords

  • CRISPR-based nucleic acid detection
  • HIV detection
  • SARS-CoV-2 detection
  • magnetofluidic separation
  • microfluidic technology

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