@article{80e73ac54bd14890bf712031dff31afd,
title = "p62(dok): A constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells",
abstract = "Characteristic of chronic myelogenous leukemia (CML) is the presence of the chimeric p210(bcr-abl) protein possessing elevated protein tyrosine kinase activity relative to normal c-abl tyrosine kinase. Hematopoietic progenitors isolated from CML patients in the chronic phase contain a constitutively tyrosine-phosphorylated protein that migrates at 62 kDa by SDS-PAGE and associates with the p120 ras GTPase-activating protein (GAP). We have purified p62(dok) from a hematopoietic cell line expressing p210(bcr- abl). p62(dok) is a novel protein with features of a signaling molecule. Association of p62(dok) with GAP correlates with its tyrosine phosphorylation. p62(dok) is rapidly tyrosine-phosphorylated upon activation of the c-Kit receptor, implicating it as a component of a signal transduction pathway downstream of receptor tyrosine kinases.",
author = "Nick Carpino and David Wisniewski and Annabel Strife and Daniel Marshak and Ryuji Kobayashi and Bruce Stillman and Bayard Clarkson",
note = "Funding Information: We thank Neena Sareen and Nick Bizios for help with the 2D gels; Jennifer Kahler, Jian Ping Liu, and Mary McBride for help with tissue culture; Nora Poppito and Camille Walker for assistance with HPLC peptide mapping and sequencing; Jacek Skowronski for the cDNA library; Dafna Bar-Sagi, Ron Pruzan, Masafumi Tanaka, Paul Kearney, and Shao Hui Zhang for advice; Nick Tonks and Dafna Bar-Sagi for reviews of the manuscript; and Jim Duffy for help with the figures. We also thank Tony Rossamondo for anti-pTyr antibody R5 and Frank McCormick for anti-GAP antibody RH6-2A. This work was supported by a National Institute of Health/National Cancer Institute grant 5P01 CA64593. ",
year = "1997",
month = jan,
day = "24",
doi = "10.1016/S0092-8674(00)81840-1",
language = "English",
volume = "88",
pages = "197--204",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "2",
}