P21-activated Kinases (PAKs) Mediate the Phosphorylation of PREX2 Protein to Initiate Feedback Inhibition of Rac1 GTPase

Douglas Barrows, Sarah M. Schoenfeld, Cindy Hodakoski, Antonina Silkov, Barry Honig, Anthony Couvillon, Aliaksei Shymanets, Bernd Nürnberg, John M. Asara, Ramon Parsons

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Background: The role of phosphorylation for regulating the Rac1 GEF PREX2 is not understood. Results: PAK phosphorylation of PREX2 downstream of PIP3 and Gβ reduces PREX2 GEF activity. Conclusion: Second messengers can initiate negative feedback to decrease Rac1 activation through PAK phosphorylation of PREX2. Significance: PAK negative regulation of GEFs could represent a broadly utilized mechanism to attenuate Rac1 activation and its outputs.

Original languageEnglish
Pages (from-to)28915-28931
Number of pages17
JournalJournal of Biological Chemistry
Volume290
Issue number48
DOIs
StatePublished - 27 Nov 2015

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