P-TEN, the tumor suppressor from human chromosome 10q23, is a dual-specificity phosphatase

Michael P. Myers, Javor P. Stolarov, Charis Eng, Jing Li, Steven I. Wang, Michael H. Wigler, Ramon Parsons, Nicholas K. Tonks

Research output: Contribution to journalArticlepeer-review

741 Scopus citations

Abstract

Protein tyrosine phosphatases (PTPs) have long been thought to play a role in tumor suppression due to their ability to antagonize the growth promoting protein tyrosine kinases. Recently, a candidate tumor suppressor from 10q23, termed P-TEN, was isolated, and sequence homology was demonstrated with members of the PTP family, as well as the cytoskeletal protein tensin. Here we show that recombinant P-TEN dephosphorylated protein and peptide substrates phosphorylated on serine, threonine, and tyrosine residues, indicating that P-TEN is a dual-specificity phosphatase. In addition, P-TEN exhibited a high degree of substrate specificity, showing selectivity for extremely acidic substrates in vitro. Furthermore, we demonstrate that mutations in P-TEN, identified from primary tumors, tumor cells lines, and a patient with Bannayan-Zonana syndrome, resulted in the ablation of phosphatase activity, demonstrating that enzymatic activity of P-TEN is necessary for its ability to function as a tumor suppressor.

Original languageEnglish
Pages (from-to)9052-9057
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number17
DOIs
StatePublished - 19 Aug 1997
Externally publishedYes

Keywords

  • Cancer
  • Protein tyrosine phosphatase
  • Signal transduction
  • Tyrosine phosphorylation

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