TY - JOUR
T1 - Ozone Therapy Protects Against Rejection in a Lung Transplantation Model
T2 - A New Treatment?
AU - Santana-Rodríguez, Norberto
AU - Llontop, Pedro
AU - Clavo, Bernardino
AU - Fiuza-Pérez, María D.
AU - Zerecero, Keila
AU - Ayub, Adil
AU - Alshehri, Khalid
AU - Yordi, Nagib A.
AU - Re, Lamberto
AU - Raad, Wissam
AU - Fernández-Pérez, Leandro
AU - García-Herrera, Ricardo
AU - Huang, Chyun Yin J.
AU - Bhora, Faiz Y.
N1 - Publisher Copyright:
© 2017 The Society of Thoracic Surgeons
PY - 2017/8
Y1 - 2017/8
N2 - Background No satisfactory treatment exists for chronic rejection (CR) after lung transplantation (LT). Our objective was to assess whether ozone (O3) treatment could ameliorate CR. Methods Male Sprague-Dawley inbred rats (n = 36) were randomly assigned into four groups: (1) control (n = 6), (2) sham (n = 6), (3) LT (n = 12), and (4) O3-LT (n = 12). Animals underwent left LT. O3 was rectally administered daily for 2 weeks before LT (from 20 to 50 μg) and 3 times/wk (50 μg/dose) up to 3 months. CR; acute rejection; and Hspb27, Prdx, Epas1, Gpx3, Vegfa, Sftpa1, Sftpb, Plvap, Klf2, Cldn5, Thbd, Dsip, Fmo2, and Sepp1 mRNA gene expression were determined. Results Severe CR was observed in all animals of LT group, but none of the O3-LT animals showed signs of CR, just a mild acute rejection was observed in 1 animal. A significant decrease of Hspb27, Prdx, Epas1, Gpx3, Vegfa, Sftpa1, Sftpb, Plvap, Klf2, Cldn5, Thbd, Dsip, and Fmo2 gene expression in the O3-LT group was observed Conclusions O3 therapy significantly delayed the onset of CR regulating the expression of genes involved in its pathogenesis. No known immunosuppressive therapy has been capable of achieving similar results. From a translational point of view, O3 therapy could become a new adjuvant treatment for CR in patients undergoing LT.
AB - Background No satisfactory treatment exists for chronic rejection (CR) after lung transplantation (LT). Our objective was to assess whether ozone (O3) treatment could ameliorate CR. Methods Male Sprague-Dawley inbred rats (n = 36) were randomly assigned into four groups: (1) control (n = 6), (2) sham (n = 6), (3) LT (n = 12), and (4) O3-LT (n = 12). Animals underwent left LT. O3 was rectally administered daily for 2 weeks before LT (from 20 to 50 μg) and 3 times/wk (50 μg/dose) up to 3 months. CR; acute rejection; and Hspb27, Prdx, Epas1, Gpx3, Vegfa, Sftpa1, Sftpb, Plvap, Klf2, Cldn5, Thbd, Dsip, Fmo2, and Sepp1 mRNA gene expression were determined. Results Severe CR was observed in all animals of LT group, but none of the O3-LT animals showed signs of CR, just a mild acute rejection was observed in 1 animal. A significant decrease of Hspb27, Prdx, Epas1, Gpx3, Vegfa, Sftpa1, Sftpb, Plvap, Klf2, Cldn5, Thbd, Dsip, and Fmo2 gene expression in the O3-LT group was observed Conclusions O3 therapy significantly delayed the onset of CR regulating the expression of genes involved in its pathogenesis. No known immunosuppressive therapy has been capable of achieving similar results. From a translational point of view, O3 therapy could become a new adjuvant treatment for CR in patients undergoing LT.
UR - http://www.scopus.com/inward/record.url?scp=85019594371&partnerID=8YFLogxK
U2 - 10.1016/j.athoracsur.2017.02.054
DO - 10.1016/j.athoracsur.2017.02.054
M3 - Article
C2 - 28549673
AN - SCOPUS:85019594371
SN - 0003-4975
VL - 104
SP - 458
EP - 464
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 2
ER -