Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells

Antonella F.M. Dost; Aaron L. Moye; Marall Vedaie; Linh M. Tran; Eileen Fung; Dar Heinze; Carlos Villacorta-Martin; Jessie Huang; Ryan Hekman; Julian H. Kwan; Benjamin C. Blum; Sharon M. Louie; Samuel P. Rowbotham; Julio Sainz de Aja; Mary E. Piper; Preetida J. Bhetariya; Roderick T. Bronson; Andrew Emili; Gustavo Mostoslavsky; Gregory A. Fishbein; William D. Wallace; Kostyantyn Krysan; Steven M. Dubinett; Jane Yanagawa; Darrell N. Kotton; Carla F. Kim

doi:10.1016/j.stem.2020.07.022

Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells

Antonella F.M. Dost
, Aaron L. Moye
, Marall Vedaie
, Linh M. Tran
, Eileen Fung
, Dar Heinze
, Carlos Villacorta-Martin
, Jessie Huang
, Ryan Hekman
, Julian H. Kwan
, Benjamin C. Blum
, Sharon M. Louie
, Samuel P. Rowbotham
, Julio Sainz de Aja
, Mary E. Piper
, Preetida J. Bhetariya
, Roderick T. Bronson
, Andrew Emili
, Gustavo Mostoslavsky
, Gregory A. Fishbein

William D. Wallace, Kostyantyn Krysan, Steven M. Dubinett, Jane Yanagawa, Darrell N. Kotton, Carla F. Kim

Research output: Contribution to journal › Article › peer-review

139 Scopus citations

Abstract

Early-stage lung cancer is poorly understood. Dost, Moye et al. introduce new organoid systems to model lung cancer. KRAS-expressing alveolar progenitor cells had reduced expression of lineage genes in mouse and organoid models and stage IA cancers. This is the first report of loss of differentiation in early-stage lung cancer.

Original language	English
Pages (from-to)	663-678.e8
Journal	Cell Stem Cell
Volume	27
Issue number	4
DOIs	https://doi.org/10.1016/j.stem.2020.07.022
State	Published - 1 Oct 2020
Externally published	Yes

Keywords

KRAS
alveolar
developmental programs
early-stage lung cancer
iPSC
loss of differentiation
organoid
single-cell RNA sequencing
stage IA lung adenocarcinoma
tumor progression

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10.1016/j.stem.2020.07.022

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Dost, A. F. M., Moye, A. L., Vedaie, M., Tran, L. M., Fung, E., Heinze, D., Villacorta-Martin, C., Huang, J., Hekman, R., Kwan, J. H., Blum, B. C., Louie, S. M., Rowbotham, S. P., Sainz de Aja, J., Piper, M. E., Bhetariya, P. J., Bronson, R. T., Emili, A., Mostoslavsky, G., ... Kim, C. F. (2020). Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells. Cell Stem Cell, 27(4), 663-678.e8. https://doi.org/10.1016/j.stem.2020.07.022

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title = "Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells",

abstract = "Early-stage lung cancer is poorly understood. Dost, Moye et al. introduce new organoid systems to model lung cancer. KRAS-expressing alveolar progenitor cells had reduced expression of lineage genes in mouse and organoid models and stage IA cancers. This is the first report of loss of differentiation in early-stage lung cancer.",

keywords = "KRAS, alveolar, developmental programs, early-stage lung cancer, iPSC, loss of differentiation, organoid, single-cell RNA sequencing, stage IA lung adenocarcinoma, tumor progression",

author = "Dost, \{Antonella F.M.\} and Moye, \{Aaron L.\} and Marall Vedaie and Tran, \{Linh M.\} and Eileen Fung and Dar Heinze and Carlos Villacorta-Martin and Jessie Huang and Ryan Hekman and Kwan, \{Julian H.\} and Blum, \{Benjamin C.\} and Louie, \{Sharon M.\} and Rowbotham, \{Samuel P.\} and \{Sainz de Aja\}, Julio and Piper, \{Mary E.\} and Bhetariya, \{Preetida J.\} and Bronson, \{Roderick T.\} and Andrew Emili and Gustavo Mostoslavsky and Fishbein, \{Gregory A.\} and Wallace, \{William D.\} and Kostyantyn Krysan and Dubinett, \{Steven M.\} and Jane Yanagawa and Kotton, \{Darrell N.\} and Kim, \{Carla F.\}",

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year = "2020",

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doi = "10.1016/j.stem.2020.07.022",

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Dost, AFM, Moye, AL, Vedaie, M, Tran, LM, Fung, E, Heinze, D, Villacorta-Martin, C, Huang, J, Hekman, R, Kwan, JH, Blum, BC, Louie, SM, Rowbotham, SP, Sainz de Aja, J, Piper, ME, Bhetariya, PJ, Bronson, RT, Emili, A, Mostoslavsky, G, Fishbein, GA, Wallace, WD, Krysan, K, Dubinett, SM, Yanagawa, J, Kotton, DN & Kim, CF 2020, 'Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells', Cell Stem Cell, vol. 27, no. 4, pp. 663-678.e8. https://doi.org/10.1016/j.stem.2020.07.022

TY - JOUR

T1 - Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells

AU - Dost, Antonella F.M.

AU - Moye, Aaron L.

AU - Vedaie, Marall

AU - Tran, Linh M.

AU - Fung, Eileen

AU - Heinze, Dar

AU - Villacorta-Martin, Carlos

AU - Huang, Jessie

AU - Hekman, Ryan

AU - Kwan, Julian H.

AU - Blum, Benjamin C.

AU - Louie, Sharon M.

AU - Rowbotham, Samuel P.

AU - Sainz de Aja, Julio

AU - Piper, Mary E.

AU - Bhetariya, Preetida J.

AU - Bronson, Roderick T.

AU - Emili, Andrew

AU - Mostoslavsky, Gustavo

AU - Fishbein, Gregory A.

AU - Wallace, William D.

AU - Krysan, Kostyantyn

AU - Dubinett, Steven M.

AU - Yanagawa, Jane

AU - Kotton, Darrell N.

AU - Kim, Carla F.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Early-stage lung cancer is poorly understood. Dost, Moye et al. introduce new organoid systems to model lung cancer. KRAS-expressing alveolar progenitor cells had reduced expression of lineage genes in mouse and organoid models and stage IA cancers. This is the first report of loss of differentiation in early-stage lung cancer.

AB - Early-stage lung cancer is poorly understood. Dost, Moye et al. introduce new organoid systems to model lung cancer. KRAS-expressing alveolar progenitor cells had reduced expression of lineage genes in mouse and organoid models and stage IA cancers. This is the first report of loss of differentiation in early-stage lung cancer.

KW - KRAS

KW - alveolar

KW - developmental programs

KW - early-stage lung cancer

KW - iPSC

KW - loss of differentiation

KW - organoid

KW - single-cell RNA sequencing

KW - stage IA lung adenocarcinoma

KW - tumor progression

UR - https://www.scopus.com/pages/publications/85091085508

U2 - 10.1016/j.stem.2020.07.022

DO - 10.1016/j.stem.2020.07.022

M3 - Article

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AN - SCOPUS:85091085508

SN - 1934-5909

VL - 27

SP - 663-678.e8

JO - Cell Stem Cell

JF - Cell Stem Cell

IS - 4

ER -

Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells

Abstract

Keywords

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