TY - JOUR
T1 - Organizing genome engineering for the gigabase scale
AU - Bartley, Bryan A.
AU - Beal, Jacob
AU - Karr, Jonathan R.
AU - Strychalski, Elizabeth A.
N1 - Funding Information:
This work was supported, in part, by NIH awards P41-EB023912 and R35-GM119771 and by NSF awards 1548123 and 1522074. We thank the reviewers for legal insights for Section “Contracts, intellectual property, and laws”, and thank Nicola Hawes for help illustrating Fig. 2. The views, opinions, and/or findings expressed are those of the author (s) and should not be interpreted as representing the official views or policies of these funding agencies or the U.S. Government. This document does not contain technology or technical data controlled under either U.S. International Traffic in Arms Regulation or U. S. Export Administration Regulations. Certain commercial equipment, instruments, or materials are identified to adequately specify experimental procedures. Such identification neither implies recommendation nor endorsement by the National Institute of Standards and Technology nor that the equipment, instruments, or materials identified are necessarily the best for the purpose.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Genome-scale engineering holds great potential to impact science, industry, medicine, and society, and recent improvements in DNA synthesis have enabled the manipulation of megabase genomes. However, coordinating and integrating the workflows and large teams necessary for gigabase genome engineering remains a considerable challenge. We examine this issue and recommend a path forward by: 1) adopting and extending existing representations for designs, assembly plans, samples, data, and workflows; 2) developing new technologies for data curation and quality control; 3) conducting fundamental research on genome-scale modeling and design; and 4) developing new legal and contractual infrastructure to facilitate collaboration.
AB - Genome-scale engineering holds great potential to impact science, industry, medicine, and society, and recent improvements in DNA synthesis have enabled the manipulation of megabase genomes. However, coordinating and integrating the workflows and large teams necessary for gigabase genome engineering remains a considerable challenge. We examine this issue and recommend a path forward by: 1) adopting and extending existing representations for designs, assembly plans, samples, data, and workflows; 2) developing new technologies for data curation and quality control; 3) conducting fundamental research on genome-scale modeling and design; and 4) developing new legal and contractual infrastructure to facilitate collaboration.
UR - http://www.scopus.com/inward/record.url?scp=85079005866&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-14314-z
DO - 10.1038/s41467-020-14314-z
M3 - Article
C2 - 32019919
AN - SCOPUS:85079005866
SN - 2041-1723
VL - 11
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 689
ER -