Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis

Ilias Alevizos; Mamatha Mahadevappa; Xue Zhang; Hiroe Ohyama; Yohko Kohno; Marshall Posner; George T. Gallagher; Mark Varvares; Donald Cohen; Dae Kim; Ralph Kent; R. Bruce Donoff; Randy Todd; Chou Ming Yung; Janet A. Warrington; David T.W. Wong

doi:10.1038/sj.onc.1204685

Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis

Ilias Alevizos
, Mamatha Mahadevappa
, Xue Zhang
, Hiroe Ohyama
, Yohko Kohno
, Marshall Posner
, George T. Gallagher
, Mark Varvares
, Donald Cohen
, Dae Kim
, Ralph Kent
, R. Bruce Donoff
, Randy Todd
, Chou Ming Yung
, Janet A. Warrington
, David T.W. Wong

Research output: Contribution to journal › Article › peer-review

203 Scopus citations

Abstract

Large scale gene expression profiling was carried out on laser capture microdissected (LCM) tumor and normal oral epithelial cells and analysed on high-density oligonucleotide microarrays. About 600 genes were found to be oral cancer associated. These oral cancer associated genes include oncogenes, tumor suppressors, transcription factors, xenobiotic enzymes, metastatic proteins, differentiation markers, and genes that have not been implicated in oral cancer. The database created provides a verifiable global profile of gene expression during oral carcinogenesis, revealing the potential role of known genes as well as genes that have not been previously implicated in oral cancer.

Original language	English
Pages (from-to)	6196-6204
Number of pages	9
Journal	Oncogene
Volume	20
Issue number	43
DOIs	https://doi.org/10.1038/sj.onc.1204685
State	Published - 27 Sep 2001
Externally published	Yes

Keywords

Gene expression
Laser capture microdissection
Microarrays
Oral cancer

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10.1038/sj.onc.1204685

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Alevizos, I., Mahadevappa, M., Zhang, X., Ohyama, H., Kohno, Y., Posner, M., Gallagher, G. T., Varvares, M., Cohen, D., Kim, D., Kent, R., Donoff, R. B., Todd, R., Yung, C. M., Warrington, J. A., & Wong, D. T. W. (2001). Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis. Oncogene, 20(43), 6196-6204. https://doi.org/10.1038/sj.onc.1204685

@article{f57fd667d376469e89cbc8e785f84433,

title = "Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis",

abstract = "Large scale gene expression profiling was carried out on laser capture microdissected (LCM) tumor and normal oral epithelial cells and analysed on high-density oligonucleotide microarrays. About 600 genes were found to be oral cancer associated. These oral cancer associated genes include oncogenes, tumor suppressors, transcription factors, xenobiotic enzymes, metastatic proteins, differentiation markers, and genes that have not been implicated in oral cancer. The database created provides a verifiable global profile of gene expression during oral carcinogenesis, revealing the potential role of known genes as well as genes that have not been previously implicated in oral cancer.",

keywords = "Gene expression, Laser capture microdissection, Microarrays, Oral cancer",

author = "Ilias Alevizos and Mamatha Mahadevappa and Xue Zhang and Hiroe Ohyama and Yohko Kohno and Marshall Posner and Gallagher, \{George T.\} and Mark Varvares and Donald Cohen and Dae Kim and Ralph Kent and Donoff, \{R. Bruce\} and Randy Todd and Yung, \{Chou Ming\} and Warrington, \{Janet A.\} and Wong, \{David T.W.\}",

note = "Funding Information: The work is supported by the National Institute of Dental and Craniofacial Research (NIDCR) grants P01 DE12467 (DTW Wong), P30 DE11814 (DTW Wong), R29 DE11983 (R Todd), Harvard University William F. Milton Fund (R Todd), Oral \& Maxillofacial Surgery Foundation (H Ohyama and R Todd), and China Site Key Basic Research Program Grant G199805123 (X Zhang). H Ohyama is supported by the Scholar in Medicine Fellowship, Harvard Medical School.",

year = "2001",

month = sep,

day = "27",

doi = "10.1038/sj.onc.1204685",

language = "English",

volume = "20",

pages = "6196--6204",

journal = "Oncogene",

issn = "0950-9232",

publisher = "Springer Nature",

number = "43",

}

Alevizos, I, Mahadevappa, M, Zhang, X, Ohyama, H, Kohno, Y, Posner, M, Gallagher, GT, Varvares, M, Cohen, D, Kim, D, Kent, R, Donoff, RB, Todd, R, Yung, CM, Warrington, JA & Wong, DTW 2001, 'Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis', Oncogene, vol. 20, no. 43, pp. 6196-6204. https://doi.org/10.1038/sj.onc.1204685

TY - JOUR

T1 - Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis

AU - Alevizos, Ilias

AU - Mahadevappa, Mamatha

AU - Zhang, Xue

AU - Ohyama, Hiroe

AU - Kohno, Yohko

AU - Posner, Marshall

AU - Gallagher, George T.

AU - Varvares, Mark

AU - Cohen, Donald

AU - Kim, Dae

AU - Kent, Ralph

AU - Donoff, R. Bruce

AU - Todd, Randy

AU - Yung, Chou Ming

AU - Warrington, Janet A.

AU - Wong, David T.W.

N1 - Funding Information: The work is supported by the National Institute of Dental and Craniofacial Research (NIDCR) grants P01 DE12467 (DTW Wong), P30 DE11814 (DTW Wong), R29 DE11983 (R Todd), Harvard University William F. Milton Fund (R Todd), Oral & Maxillofacial Surgery Foundation (H Ohyama and R Todd), and China Site Key Basic Research Program Grant G199805123 (X Zhang). H Ohyama is supported by the Scholar in Medicine Fellowship, Harvard Medical School.

PY - 2001/9/27

Y1 - 2001/9/27

N2 - Large scale gene expression profiling was carried out on laser capture microdissected (LCM) tumor and normal oral epithelial cells and analysed on high-density oligonucleotide microarrays. About 600 genes were found to be oral cancer associated. These oral cancer associated genes include oncogenes, tumor suppressors, transcription factors, xenobiotic enzymes, metastatic proteins, differentiation markers, and genes that have not been implicated in oral cancer. The database created provides a verifiable global profile of gene expression during oral carcinogenesis, revealing the potential role of known genes as well as genes that have not been previously implicated in oral cancer.

AB - Large scale gene expression profiling was carried out on laser capture microdissected (LCM) tumor and normal oral epithelial cells and analysed on high-density oligonucleotide microarrays. About 600 genes were found to be oral cancer associated. These oral cancer associated genes include oncogenes, tumor suppressors, transcription factors, xenobiotic enzymes, metastatic proteins, differentiation markers, and genes that have not been implicated in oral cancer. The database created provides a verifiable global profile of gene expression during oral carcinogenesis, revealing the potential role of known genes as well as genes that have not been previously implicated in oral cancer.

KW - Gene expression

KW - Laser capture microdissection

KW - Microarrays

KW - Oral cancer

UR - https://www.scopus.com/pages/publications/0035959741

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VL - 20

SP - 6196

EP - 6204

JO - Oncogene

JF - Oncogene

IS - 43

ER -

Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis

Abstract

Keywords

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