Optimized β-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos

Satoshi Kishigami; Yoshihiro Komatsu; Haruko Takeda; Aya Nomura-Kitabayashi; Yasutaka Yamauchi; Kuniya Abe; Ken Ichi Yamamura; Yuji Mishina

doi:10.1002/gene.20186

Optimized β-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos

Satoshi Kishigami
, Yoshihiro Komatsu
, Haruko Takeda
, Aya Nomura-Kitabayashi
, Yasutaka Yamauchi
, Kuniya Abe
, Ken Ichi Yamamura
, Yuji Mishina

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

β-Galactosidase (β-gal) is one of the popular reporters for detecting the expression of endogenous or exogenous genes. Here we report 6-chloro-3-indoxyl-beta-D-galactopyranoside (S-gal) is more sensitive for β-gal activity than 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal), particularly during the early developmental stages of mouse embryos. Further, we successfully combined β-gal staining with S-gal and in situ hybridization using DIG-labeled probes in both whole and sections of early stage embryos due to the sensitivity and color compatibility of S-gal.

Original language	English
Pages (from-to)	57-65
Number of pages	9
Journal	Genesis (United States)
Volume	44
Issue number	2
DOIs	https://doi.org/10.1002/gene.20186
State	Published - Feb 2006
Externally published	Yes

Keywords

Beta-galactosidase
In situ hybridization
S-gal
X-gal

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10.1002/gene.20186

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title = "Optimized β-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos",

abstract = "β-Galactosidase (β-gal) is one of the popular reporters for detecting the expression of endogenous or exogenous genes. Here we report 6-chloro-3-indoxyl-beta-D-galactopyranoside (S-gal) is more sensitive for β-gal activity than 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal), particularly during the early developmental stages of mouse embryos. Further, we successfully combined β-gal staining with S-gal and in situ hybridization using DIG-labeled probes in both whole and sections of early stage embryos due to the sensitivity and color compatibility of S-gal.",

keywords = "Beta-galactosidase, In situ hybridization, S-gal, X-gal",

author = "Satoshi Kishigami and Yoshihiro Komatsu and Haruko Takeda and Aya Nomura-Kitabayashi and Yasutaka Yamauchi and Kuniya Abe and Yamamura, \{Ken Ichi\} and Yuji Mishina",

year = "2006",

month = feb,

doi = "10.1002/gene.20186",

language = "English",

volume = "44",

pages = "57--65",

journal = "Genesis (United States)",

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TY - JOUR

T1 - Optimized β-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos

AU - Kishigami, Satoshi

AU - Komatsu, Yoshihiro

AU - Takeda, Haruko

AU - Nomura-Kitabayashi, Aya

AU - Yamauchi, Yasutaka

AU - Abe, Kuniya

AU - Yamamura, Ken Ichi

AU - Mishina, Yuji

PY - 2006/2

Y1 - 2006/2

N2 - β-Galactosidase (β-gal) is one of the popular reporters for detecting the expression of endogenous or exogenous genes. Here we report 6-chloro-3-indoxyl-beta-D-galactopyranoside (S-gal) is more sensitive for β-gal activity than 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal), particularly during the early developmental stages of mouse embryos. Further, we successfully combined β-gal staining with S-gal and in situ hybridization using DIG-labeled probes in both whole and sections of early stage embryos due to the sensitivity and color compatibility of S-gal.

AB - β-Galactosidase (β-gal) is one of the popular reporters for detecting the expression of endogenous or exogenous genes. Here we report 6-chloro-3-indoxyl-beta-D-galactopyranoside (S-gal) is more sensitive for β-gal activity than 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal), particularly during the early developmental stages of mouse embryos. Further, we successfully combined β-gal staining with S-gal and in situ hybridization using DIG-labeled probes in both whole and sections of early stage embryos due to the sensitivity and color compatibility of S-gal.

KW - Beta-galactosidase

KW - In situ hybridization

KW - S-gal

KW - X-gal

UR - https://www.scopus.com/pages/publications/33645214464

U2 - 10.1002/gene.20186

DO - 10.1002/gene.20186

M3 - Article

C2 - 16419090

AN - SCOPUS:33645214464

SN - 1526-954X

VL - 44

SP - 57

EP - 65

JO - Genesis (United States)

JF - Genesis (United States)

IS - 2

ER -

Optimized β-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos

Abstract

Keywords

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