Abstract
β-Galactosidase (β-gal) is one of the popular reporters for detecting the expression of endogenous or exogenous genes. Here we report 6-chloro-3-indoxyl-beta-D-galactopyranoside (S-gal) is more sensitive for β-gal activity than 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal), particularly during the early developmental stages of mouse embryos. Further, we successfully combined β-gal staining with S-gal and in situ hybridization using DIG-labeled probes in both whole and sections of early stage embryos due to the sensitivity and color compatibility of S-gal.
Original language | English |
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Pages (from-to) | 57-65 |
Number of pages | 9 |
Journal | Genesis (United States) |
Volume | 44 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2006 |
Externally published | Yes |
Keywords
- Beta-galactosidase
- In situ hybridization
- S-gal
- X-gal