Optimized β-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos

Satoshi Kishigami, Yoshihiro Komatsu, Haruko Takeda, Aya Nomura-Kitabayashi, Yasutaka Yamauchi, Kuniya Abe, Ken Ichi Yamamura, Yuji Mishina

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

β-Galactosidase (β-gal) is one of the popular reporters for detecting the expression of endogenous or exogenous genes. Here we report 6-chloro-3-indoxyl-beta-D-galactopyranoside (S-gal) is more sensitive for β-gal activity than 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal), particularly during the early developmental stages of mouse embryos. Further, we successfully combined β-gal staining with S-gal and in situ hybridization using DIG-labeled probes in both whole and sections of early stage embryos due to the sensitivity and color compatibility of S-gal.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalGenesis (United States)
Volume44
Issue number2
DOIs
StatePublished - Feb 2006
Externally publishedYes

Keywords

  • Beta-galactosidase
  • In situ hybridization
  • S-gal
  • X-gal

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