Optimization of Mycelial Growth Parameters in Lion’s Mane Edible Medicinal Mushroom Hericium erinaceus (Agaricomycetes) by Supplementation of Growth Hormones

The present study was carried out to optimize the strain and evaluate the effect of amendment of growth media with different hormone concentrations for enhancing mycelium growth of lion’s mane mushroom Hericium erinaceus under in vitro conditions. Among the five strains of H. erinaceus, He-04 strain showed maximum average GR (GRavr) of 4.78 mm d^–1. Five different media, potato dextrose agar (PDA), malt extract agar, sawdust extract agar, wheat straw extract agar, and rice straw extract agar, amended with four concentrations (10, 20, 30, and 40 ppm) of gibberellic acid, kinetin, and indole acetic acid, were evaluated for promotion of mycelial growth of H. erinaceus. PDA was observed to be the best media promoting the mycelial growth of H. erinaceus. The highest mycelial GRavr 8.47 mm d^–1 was observed in PDA amended with indole acetic acid (10 ppm) followed by gibberellic acid and kinetin (30 ppm) decreasing mycelial GRav to 8.15 and 7.75mm d^–1, respectively. Temperature of 25°C and pH 7.0 was found to be the best for mycelium growth of H. erinaceus.