Optimisation of the degenerate oligonucleotide primed PCR (DOP-PCR) for capillary thermocycler

Csaba Kiss, Maria Kost-Alimova, George Klein, Laszlo Szekely

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

DOP-PCR was optimised for capillary thermocycler. Five primers with five or six nucleotide anchor sequences and different restriction enzyme recognition sites were tested. The efficiency of the amplification was confirmed by gel electrophoresis and fluorescence in situ hybridisation on metaphase chromosomes.

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalBiomolecular Engineering
Volume19
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • Capillary PCR
  • DOP-PCR
  • PCR optimisation

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