Optimal protocol for the differentiation and metabolic analysis of human adipose stromal cells

Mi Jeong Lee, Susan K. Fried

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

51 Scopus citations

Abstract

Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be isolated from distinct adipose depots and cultured in vitro, have clear translational relevance. We describe the methods to isolate, culture, and differentiate human ASCs to adipocytes that respond to physiologically relevant hormones, such as insulin and β-adrenergic agonists. We also describe methods for assaying hormonal effects on glucose transport and lipolysis.

Original languageEnglish
Title of host publicationMethods of Adipose Tissue Biology, Part B
PublisherAcademic Press Inc.
Pages49-65
Number of pages17
ISBN (Print)9780128002803
DOIs
StatePublished - 2014
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume538
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Adipocytes
  • Adipogenesis
  • Adipose tissue
  • Glucose transport
  • Lipolysis
  • Preadipocyte

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