@inbook{8a79563f66de4e5aafb64f90fe14ae83,
title = "One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan",
abstract = "Heparin, a glycosaminoglycan-based anticoagulant drug, is prepared as an extract of animal tissues. Heparosan, an Escherichia coli (E. coli) K5 capsular polysaccharide with the structure →4)-β-D-glucuronic acid (1 → 4)-β-D-N-acetylglucosamine (1→, corresponds to the precursor backbone in the Golgi-based biosynthesis of heparin. Anticoagulant heparin is prepared in a one-pot synthesis using a chemically prepared derivative of heparosan called N-sulfoheparosan (NSH), recombinant Golgi enzymes expressed in E. coli, and the 3-phosphoadenosine-5-phosphosulfate (PAPS) cofactor.",
keywords = "3-Phosphoadenosine-5-phosphosulfate, Biosynthesis, Enzymes, Heparan sulfate, Heparin, Heparosan",
author = "Li Fu and Linhardt, {Robert J.}",
note = "Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1398-6_1",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "3--11",
booktitle = "Methods in Molecular Biology",
}