Omics era in stem cell research: Data integration of multi-regulatory layers

Ana Sevilla, Benjamin Trinite, Ihor R. Lemischka

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Embryonic stem cells have two unique properties: self-renewal and pluripotency (Chen and Daley, Hum Mol Genet 17:R23-R27, 2008; Jaenisch and Young, Cell 132:567-582, 2008). Self-renewal allows stem cells to generate unlimited copies of them, whereas pluripotency is the capacity to differentiate into any tissue. These two properties make embryonic stem cell research exciting for potential use in therapeutic applications (Murry and Keller, Cell 132:661-680, 2008). The transition of embryonic stem cells, from an uncommitted state to a differentiated state, involves the global execution of specific programs requiring changes in the various intra-cellular regulatory layers involving the transcriptome, the proteome, the epigenome and the methylome. During these changes, pluripotency factors are silenced and lineage-specific programs are activated in an orchestrated fashion. In this chapter we review recent efforts of studying the different regulatory layers involved in embryonic stem cells early differentiation. We describe in detail different techniques used for analysing of each regulatory layer. These techniques involve the handling of large amount of data and require the use of powerful informatics tools.

Original languageEnglish
Title of host publicationNew Frontiers of Network Analysis in Systems Biology
PublisherSpringer Netherlands
Pages119-137
Number of pages19
ISBN (Electronic)9789400743304
ISBN (Print)9400743297, 9789400743298
DOIs
StatePublished - 1 Feb 2012

Keywords

  • Bioinformatics tools
  • Epigenome
  • Methylome
  • Proteome
  • Regulatory layers
  • Stem cells systems biology
  • Transcriptome

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