TY - JOUR
T1 - O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity
AU - Florman, Harvey M.
AU - Wassarman, Paul M.
N1 - Funding Information:
We are grateful to Dr. John H. Elder for a generous gift of endoglycosi-dase F and to the members of our laboratory for advice and constructive criticism throughout the course of this research. The research was supported in part by a grant from the National Institute of Child Health and Human Development (HD-12275) awarded to P M. W. H. M. F. is a National Institutes of Health postdoctoral fellow.
PY - 1985/5
Y1 - 1985/5
N2 - Previously, we reported that ZP3, one of three different glycoproteins present in the mouse egg's zona pellucida, serves as a sperm receptor. Furthermore, small glycopeptides derived from egg ZP3 retain full sperm receptor activity, suggesting a role for carbohydrate, rather than polypeptide chain in receptor function. Here, we report that removal of O-linked oligosaccharides from ZP3 destroys its sperm receptor activity, whereas removal of O-linked oligosaccharides has no effect. A specific size class of O-linked oligosaccharides, recovered following mild alkaline hydrolysis and reduction of ZP3, is shown to possess sperm receptor activity and to bind to sperm. The results presented strongly suggest that mouse sperm bind to eggs via O-linked oligosaccharides present on ZP3.
AB - Previously, we reported that ZP3, one of three different glycoproteins present in the mouse egg's zona pellucida, serves as a sperm receptor. Furthermore, small glycopeptides derived from egg ZP3 retain full sperm receptor activity, suggesting a role for carbohydrate, rather than polypeptide chain in receptor function. Here, we report that removal of O-linked oligosaccharides from ZP3 destroys its sperm receptor activity, whereas removal of O-linked oligosaccharides has no effect. A specific size class of O-linked oligosaccharides, recovered following mild alkaline hydrolysis and reduction of ZP3, is shown to possess sperm receptor activity and to bind to sperm. The results presented strongly suggest that mouse sperm bind to eggs via O-linked oligosaccharides present on ZP3.
UR - http://www.scopus.com/inward/record.url?scp=0021874149&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(85)90084-4
DO - 10.1016/0092-8674(85)90084-4
M3 - Article
C2 - 2986849
AN - SCOPUS:0021874149
SN - 0092-8674
VL - 41
SP - 313
EP - 324
JO - Cell
JF - Cell
IS - 1
ER -