Nucleotide sequence analysis identifies the human c-sis proto-oncogene as a structural gene for platelet-derived growth factor

E. Ing-Ming Chiu; Premkumar Reddy; David Givol; Keith C. Robbins; Steven R. Tronick; Stuart A. Aaronson

doi:10.1016/0092-8674(84)90307-6

Nucleotide sequence analysis identifies the human c-sis proto-oncogene as a structural gene for platelet-derived growth factor

E. Ing-Ming Chiu, Premkumar Reddy, David Givol, Keith C. Robbins, Steven R. Tronick, Stuart A. Aaronson

Research output: Contribution to journal › Article › peer-review

114 Scopus citations

Abstract

The simian sarcoma virus transforming gene, v-sis, encodes a protein, p28^sis, that is closely related to human platelet-derived growth factor (PDGF). The human locus related to v-sis was cloned and shown to contain at least five exons corresponding to the v-sis coding region. Nucleotide sequence analysis of these exons revealed that the predicted amino acid sequence of human c-sis differed by 6% from that of the woolly monkey-derived v-sis. These findings imply that the sis proto-oncogene has been well conserved during primate evolution. By comparison of the known amino acid sequences of PDGF peptides with the predicted human c-sis protein, it was possible to demonstrate that this human proto-oncogene is the structural gene encoding one of the two major polypeptides of this potent mitogen for connective tissue cells.

Original language	English
Pages (from-to)	123-129
Number of pages	7
Journal	Cell
Volume	37
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(84)90307-6
State	Published - May 1984
Externally published	Yes

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title = "Nucleotide sequence analysis identifies the human c-sis proto-oncogene as a structural gene for platelet-derived growth factor",

abstract = "The simian sarcoma virus transforming gene, v-sis, encodes a protein, p28sis, that is closely related to human platelet-derived growth factor (PDGF). The human locus related to v-sis was cloned and shown to contain at least five exons corresponding to the v-sis coding region. Nucleotide sequence analysis of these exons revealed that the predicted amino acid sequence of human c-sis differed by 6% from that of the woolly monkey-derived v-sis. These findings imply that the sis proto-oncogene has been well conserved during primate evolution. By comparison of the known amino acid sequences of PDGF peptides with the predicted human c-sis protein, it was possible to demonstrate that this human proto-oncogene is the structural gene encoding one of the two major polypeptides of this potent mitogen for connective tissue cells.",

author = "{Ing-Ming Chiu}, E. and Premkumar Reddy and David Givol and Robbins, {Keith C.} and Tronick, {Steven R.} and Aaronson, {Stuart A.}",

year = "1984",

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T1 - Nucleotide sequence analysis identifies the human c-sis proto-oncogene as a structural gene for platelet-derived growth factor

AU - Ing-Ming Chiu, E.

AU - Reddy, Premkumar

AU - Givol, David

AU - Robbins, Keith C.

AU - Tronick, Steven R.

AU - Aaronson, Stuart A.

PY - 1984/5

Y1 - 1984/5

N2 - The simian sarcoma virus transforming gene, v-sis, encodes a protein, p28sis, that is closely related to human platelet-derived growth factor (PDGF). The human locus related to v-sis was cloned and shown to contain at least five exons corresponding to the v-sis coding region. Nucleotide sequence analysis of these exons revealed that the predicted amino acid sequence of human c-sis differed by 6% from that of the woolly monkey-derived v-sis. These findings imply that the sis proto-oncogene has been well conserved during primate evolution. By comparison of the known amino acid sequences of PDGF peptides with the predicted human c-sis protein, it was possible to demonstrate that this human proto-oncogene is the structural gene encoding one of the two major polypeptides of this potent mitogen for connective tissue cells.

AB - The simian sarcoma virus transforming gene, v-sis, encodes a protein, p28sis, that is closely related to human platelet-derived growth factor (PDGF). The human locus related to v-sis was cloned and shown to contain at least five exons corresponding to the v-sis coding region. Nucleotide sequence analysis of these exons revealed that the predicted amino acid sequence of human c-sis differed by 6% from that of the woolly monkey-derived v-sis. These findings imply that the sis proto-oncogene has been well conserved during primate evolution. By comparison of the known amino acid sequences of PDGF peptides with the predicted human c-sis protein, it was possible to demonstrate that this human proto-oncogene is the structural gene encoding one of the two major polypeptides of this potent mitogen for connective tissue cells.

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Nucleotide sequence analysis identifies the human c-sis proto-oncogene as a structural gene for platelet-derived growth factor

Abstract

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