Nuclear magnetic resonance quantification for monitoring heparosan K5 capsular polysaccharide production

Zhenyu Wang, Zhenqing Zhang, Scott A. McCallum, Robert J. Linhardt

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Traditional chromatographic quantification methods for heparosan produced from the Escherichia coli K5 strain rely on extensive purification requiring laborious sample preparation. These methods are time-consuming, often resulting in sample loss during purification, and thus might not accurately reflect the amount of heparosan in the original mixture. A simple, sensitive 1H nuclear magnetic resonance (NMR) quantification method that directly quantifies heparosan K5 polysaccharide present in E. coli fermentation supernatant is described.

Original languageEnglish
Pages (from-to)275-277
Number of pages3
JournalAnalytical Biochemistry
Volume398
Issue number2
DOIs
StatePublished - Mar 2010
Externally publishedYes

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