Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation

M. Kramski, A. J. Gaeguta, G. F. Lichtfuss, R. Rajasuriar, S. M. Crowe, M. A. French, S. R. Lewin, R. J. Center, D. F.J. Purcell

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.

Original languageEnglish
Pages (from-to)3691-3693
Number of pages3
JournalJournal of Clinical Microbiology
Volume49
Issue number10
DOIs
StatePublished - Oct 2011
Externally publishedYes

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