Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation

M. Kramski; A. J. Gaeguta; G. F. Lichtfuss; R. Rajasuriar; S. M. Crowe; M. A. French; S. R. Lewin; R. J. Center; D. F.J. Purcell

doi:10.1128/JCM.01018-11

Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation

M. Kramski, A. J. Gaeguta, G. F. Lichtfuss, R. Rajasuriar, S. M. Crowe, M. A. French, S. R. Lewin, R. J. Center, D. F.J. Purcell

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Abstract

We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.

Original language	English
Pages (from-to)	3691-3693
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	10
DOIs	https://doi.org/10.1128/JCM.01018-11
State	Published - Oct 2011
Externally published	Yes

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Kramski, M., Gaeguta, A. J., Lichtfuss, G. F., Rajasuriar, R., Crowe, S. M., French, M. A., Lewin, S. R., Center, R. J., & Purcell, D. F. J. (2011). Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation. Journal of Clinical Microbiology, 49(10), 3691-3693. https://doi.org/10.1128/JCM.01018-11

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abstract = "We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.",

author = "M. Kramski and Gaeguta, {A. J.} and Lichtfuss, {G. F.} and R. Rajasuriar and Crowe, {S. M.} and French, {M. A.} and Lewin, {S. R.} and Center, {R. J.} and Purcell, {D. F.J.}",

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doi = "10.1128/JCM.01018-11",

language = "English",

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Kramski, M, Gaeguta, AJ, Lichtfuss, GF, Rajasuriar, R, Crowe, SM, French, MA, Lewin, SR, Center, RJ & Purcell, DFJ 2011, 'Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation', Journal of Clinical Microbiology, vol. 49, no. 10, pp. 3691-3693. https://doi.org/10.1128/JCM.01018-11

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AU - Gaeguta, A. J.

AU - Lichtfuss, G. F.

AU - Rajasuriar, R.

AU - Crowe, S. M.

AU - French, M. A.

AU - Lewin, S. R.

AU - Center, R. J.

AU - Purcell, D. F.J.

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Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation

Abstract

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