Novel protein crystal growth electrochemical cell for applications in x-ray diffraction and atomic force microscopy

Gabriela Gil-Alvaradejo; Rayana R. Ruiz-Arellano; Christopher Owen; Adela Rodríguez-Romero; Enrique Rudiño-Piñera; Moriamou K. Antwi; Vivian Stojanoff; Abel Moreno

doi:10.1021/cg200485v

Novel protein crystal growth electrochemical cell for applications in x-ray diffraction and atomic force microscopy

Gabriela Gil-Alvaradejo, Rayana R. Ruiz-Arellano, Christopher Owen, Adela Rodríguez-Romero, Enrique Rudiño-Piñera, Moriamou K. Antwi, Vivian Stojanoff, Abel Moreno

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A new crystal growth cell based on transparent indium tin oxide (ITO) glass-electrodes for electrochemically assisted protein crystallization allows for reduced nucleation and crystal quality enhancement. The crystallization behavior of lysozyme and ferritin was monitored as a function of the electric current applied to the growth cell. The X-ray diffraction analysis showed that for specific currents, the crystal quality is substantially improved. No conformational changes were observed in the 3D crystallographic structures determined for crystals grown under different electric current regimes. Finally, the strong crystal adhesion on the surface of ITO electrode because of the electroadhesion allows a sufficiently strong fixing of the protein crystals, to undergo atomic force microscopy investigations in a fluid cell.

Original language	English
Pages (from-to)	3917-3922
Number of pages	6
Journal	Crystal Growth and Design
Volume	11
Issue number	9
DOIs	https://doi.org/10.1021/cg200485v
State	Published - 7 Sep 2011
Externally published	Yes

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title = "Novel protein crystal growth electrochemical cell for applications in x-ray diffraction and atomic force microscopy",

abstract = "A new crystal growth cell based on transparent indium tin oxide (ITO) glass-electrodes for electrochemically assisted protein crystallization allows for reduced nucleation and crystal quality enhancement. The crystallization behavior of lysozyme and ferritin was monitored as a function of the electric current applied to the growth cell. The X-ray diffraction analysis showed that for specific currents, the crystal quality is substantially improved. No conformational changes were observed in the 3D crystallographic structures determined for crystals grown under different electric current regimes. Finally, the strong crystal adhesion on the surface of ITO electrode because of the electroadhesion allows a sufficiently strong fixing of the protein crystals, to undergo atomic force microscopy investigations in a fluid cell.",

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T1 - Novel protein crystal growth electrochemical cell for applications in x-ray diffraction and atomic force microscopy

AU - Gil-Alvaradejo, Gabriela

AU - Ruiz-Arellano, Rayana R.

AU - Owen, Christopher

AU - Rodríguez-Romero, Adela

AU - Rudiño-Piñera, Enrique

AU - Antwi, Moriamou K.

AU - Stojanoff, Vivian

AU - Moreno, Abel

PY - 2011/9/7

Y1 - 2011/9/7

N2 - A new crystal growth cell based on transparent indium tin oxide (ITO) glass-electrodes for electrochemically assisted protein crystallization allows for reduced nucleation and crystal quality enhancement. The crystallization behavior of lysozyme and ferritin was monitored as a function of the electric current applied to the growth cell. The X-ray diffraction analysis showed that for specific currents, the crystal quality is substantially improved. No conformational changes were observed in the 3D crystallographic structures determined for crystals grown under different electric current regimes. Finally, the strong crystal adhesion on the surface of ITO electrode because of the electroadhesion allows a sufficiently strong fixing of the protein crystals, to undergo atomic force microscopy investigations in a fluid cell.

AB - A new crystal growth cell based on transparent indium tin oxide (ITO) glass-electrodes for electrochemically assisted protein crystallization allows for reduced nucleation and crystal quality enhancement. The crystallization behavior of lysozyme and ferritin was monitored as a function of the electric current applied to the growth cell. The X-ray diffraction analysis showed that for specific currents, the crystal quality is substantially improved. No conformational changes were observed in the 3D crystallographic structures determined for crystals grown under different electric current regimes. Finally, the strong crystal adhesion on the surface of ITO electrode because of the electroadhesion allows a sufficiently strong fixing of the protein crystals, to undergo atomic force microscopy investigations in a fluid cell.

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Novel protein crystal growth electrochemical cell for applications in x-ray diffraction and atomic force microscopy

Abstract

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