TY - JOUR
T1 - Novel bat influenza virus NS1 proteins bind double-stranded RNA and antagonize host innate immunity
AU - Turkington, Hannah L.
AU - Juozapaitis, Mindaugas
AU - Kerry, Philip S.
AU - Aydillo, Teresa
AU - Ayllon, Juan
AU - García-Sastre, Adolfo
AU - Schwemmle, Martin
AU - Hale, Benjamin G.
PY - 2015
Y1 - 2015
N2 - We demonstrate that novel bat HL17NL10 and HL18NL11 influenza virus NS1 proteins are effective interferon antagonists but do not block general host gene expression. Solving the RNA-binding domain structures revealed the canonical NS1 symmetrical homodimer, and RNA binding required conserved basic residues in this domain. Interferon antagonism was strictly dependent on RNA binding, and chimeric bat influenza viruses expressing NS1s defective in this activity were highly attenuated in interferon- competent cells but not in cells unable to establish antiviral immunity.
AB - We demonstrate that novel bat HL17NL10 and HL18NL11 influenza virus NS1 proteins are effective interferon antagonists but do not block general host gene expression. Solving the RNA-binding domain structures revealed the canonical NS1 symmetrical homodimer, and RNA binding required conserved basic residues in this domain. Interferon antagonism was strictly dependent on RNA binding, and chimeric bat influenza viruses expressing NS1s defective in this activity were highly attenuated in interferon- competent cells but not in cells unable to establish antiviral immunity.
UR - http://www.scopus.com/inward/record.url?scp=84942155957&partnerID=8YFLogxK
U2 - 10.1128/JVI.01430-15
DO - 10.1128/JVI.01430-15
M3 - Article
C2 - 26246567
AN - SCOPUS:84942155957
SN - 0022-538X
VL - 89
SP - 10696
EP - 10701
JO - Journal of Virology
JF - Journal of Virology
IS - 20
ER -