Nonviral transfection of distinct types of human dendritic cells: High-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells

V. F.I. Van Tendeloo; H. W. Snoeck; F. Lardon; G. L.E.E. Vanham; G. Nijs; M. Lenjou; L. Hendriks; C. Van Broeckhoven; A. Moulijn; I. Rodrigus; P. Verdonk; D. R. Van Bockstaele; Z. N. Berneman

doi:10.1038/sj.gt.3300626

Nonviral transfection of distinct types of human dendritic cells: High-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells

V. F.I. Van Tendeloo, H. W. Snoeck, F. Lardon, G. L.E.E. Vanham, G. Nijs, M. Lenjou, L. Hendriks, C. Van Broeckhoven, A. Moulijn, I. Rodrigus, P. Verdonk, D. R. Van Bockstaele, Z. N. Berneman

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

Human dendritic cells (DC) are highly professional antigen presenting cells for the priming of naive cytotoxic T cells. Gene transfer in DC would be a useful strategy to load DC with relevant de novo synthesized antigens for immunotherapeutical purposes. As a first step towards a DC-based gene therapy, we examined the efficiency of nonviral transfection in different types of cultured human dendritic cells with a humanized red-shifted green fluorescent protein reporter gene. Plasmid DNA transfection by electroporation or lipofection was used to transfect CD34⁺ progenitor cell-derived DC (PC-DC) and Langerhans' cells (PC-LC), as well as monocyte-derived DC (Mo-DC). While lipofection was unsuccessful in all types of DC, we obtained high-efficiency gene transfer by electroporation in PC-LC (16%) and PC-DC (12%). In contrast, electroporation was strikingly less efficient in Mo-DC (≤ 2%). The potent allostimulatory capacity of DC was still retained in electroporated PC-DC and PC-LC. In conclusion, electroporation of antigen expressing plasmid DNA is an efficient tool for nonviral gene transfer in PC-DC and PC-LC, but not in Mo-DC and could be useful for the development of DC-based tumor immunotherapy.

Original language	English
Pages (from-to)	700-707
Number of pages	8
Journal	Gene Therapy
Volume	5
Issue number	5
DOIs	https://doi.org/10.1038/sj.gt.3300626
State	Published - 1998
Externally published	Yes

Keywords

Dendritic cells
Electroporation
Flow cytometry
Gene transfer
Green fluorescent protein
Transfection efficiency

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Van Tendeloo, V. F. I., Snoeck, H. W., Lardon, F., Vanham, G. L. E. E., Nijs, G., Lenjou, M., Hendriks, L., Van Broeckhoven, C., Moulijn, A., Rodrigus, I., Verdonk, P., Van Bockstaele, D. R., & Berneman, Z. N. (1998). Nonviral transfection of distinct types of human dendritic cells: High-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells. Gene Therapy, 5(5), 700-707. https://doi.org/10.1038/sj.gt.3300626

@article{220766896da74677958fe99c3a72f79f,

title = "Nonviral transfection of distinct types of human dendritic cells: High-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells",

abstract = "Human dendritic cells (DC) are highly professional antigen presenting cells for the priming of naive cytotoxic T cells. Gene transfer in DC would be a useful strategy to load DC with relevant de novo synthesized antigens for immunotherapeutical purposes. As a first step towards a DC-based gene therapy, we examined the efficiency of nonviral transfection in different types of cultured human dendritic cells with a humanized red-shifted green fluorescent protein reporter gene. Plasmid DNA transfection by electroporation or lipofection was used to transfect CD34+ progenitor cell-derived DC (PC-DC) and Langerhans' cells (PC-LC), as well as monocyte-derived DC (Mo-DC). While lipofection was unsuccessful in all types of DC, we obtained high-efficiency gene transfer by electroporation in PC-LC (16%) and PC-DC (12%). In contrast, electroporation was strikingly less efficient in Mo-DC (≤ 2%). The potent allostimulatory capacity of DC was still retained in electroporated PC-DC and PC-LC. In conclusion, electroporation of antigen expressing plasmid DNA is an efficient tool for nonviral gene transfer in PC-DC and PC-LC, but not in Mo-DC and could be useful for the development of DC-based tumor immunotherapy.",

keywords = "Dendritic cells, Electroporation, Flow cytometry, Gene transfer, Green fluorescent protein, Transfection efficiency",

author = "{Van Tendeloo}, {V. F.I.} and Snoeck, {H. W.} and F. Lardon and Vanham, {G. L.E.E.} and G. Nijs and M. Lenjou and L. Hendriks and {Van Broeckhoven}, C. and A. Moulijn and I. Rodrigus and P. Verdonk and {Van Bockstaele}, {D. R.} and Berneman, {Z. N.}",

note = "Funding Information: This work was supported by grants Nos 3.0109.96, 3.0226.96 and S 2/5-AV.-E106 of the Fund for Scientific Research, Flanders, Belgium (FWO), by a grant of the Scientific Committee of the ASLK-financed Cancer Research and by gifts from Mrs Ellie Van Belleghem to the HEBA Foundation (Foundation for Hematology, Bone Marrow Transplantation and Blood Transfusion Antwerp) in memory of her late mother Louisa De Saede-leer. VVT is a research assistant of the FWO.",

year = "1998",

doi = "10.1038/sj.gt.3300626",

language = "English",

volume = "5",

pages = "700--707",

journal = "Gene Therapy",

issn = "0969-7128",

publisher = "Springer Nature",

number = "5",

}

Van Tendeloo, VFI, Snoeck, HW, Lardon, F, Vanham, GLEE, Nijs, G, Lenjou, M, Hendriks, L, Van Broeckhoven, C, Moulijn, A, Rodrigus, I, Verdonk, P, Van Bockstaele, DR & Berneman, ZN 1998, 'Nonviral transfection of distinct types of human dendritic cells: High-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells', Gene Therapy, vol. 5, no. 5, pp. 700-707. https://doi.org/10.1038/sj.gt.3300626

TY - JOUR

T1 - Nonviral transfection of distinct types of human dendritic cells

T2 - High-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells

AU - Van Tendeloo, V. F.I.

AU - Snoeck, H. W.

AU - Lardon, F.

AU - Vanham, G. L.E.E.

AU - Nijs, G.

AU - Lenjou, M.

AU - Hendriks, L.

AU - Van Broeckhoven, C.

AU - Moulijn, A.

AU - Rodrigus, I.

AU - Verdonk, P.

AU - Van Bockstaele, D. R.

AU - Berneman, Z. N.

N1 - Funding Information: This work was supported by grants Nos 3.0109.96, 3.0226.96 and S 2/5-AV.-E106 of the Fund for Scientific Research, Flanders, Belgium (FWO), by a grant of the Scientific Committee of the ASLK-financed Cancer Research and by gifts from Mrs Ellie Van Belleghem to the HEBA Foundation (Foundation for Hematology, Bone Marrow Transplantation and Blood Transfusion Antwerp) in memory of her late mother Louisa De Saede-leer. VVT is a research assistant of the FWO.

PY - 1998

Y1 - 1998

N2 - Human dendritic cells (DC) are highly professional antigen presenting cells for the priming of naive cytotoxic T cells. Gene transfer in DC would be a useful strategy to load DC with relevant de novo synthesized antigens for immunotherapeutical purposes. As a first step towards a DC-based gene therapy, we examined the efficiency of nonviral transfection in different types of cultured human dendritic cells with a humanized red-shifted green fluorescent protein reporter gene. Plasmid DNA transfection by electroporation or lipofection was used to transfect CD34+ progenitor cell-derived DC (PC-DC) and Langerhans' cells (PC-LC), as well as monocyte-derived DC (Mo-DC). While lipofection was unsuccessful in all types of DC, we obtained high-efficiency gene transfer by electroporation in PC-LC (16%) and PC-DC (12%). In contrast, electroporation was strikingly less efficient in Mo-DC (≤ 2%). The potent allostimulatory capacity of DC was still retained in electroporated PC-DC and PC-LC. In conclusion, electroporation of antigen expressing plasmid DNA is an efficient tool for nonviral gene transfer in PC-DC and PC-LC, but not in Mo-DC and could be useful for the development of DC-based tumor immunotherapy.

AB - Human dendritic cells (DC) are highly professional antigen presenting cells for the priming of naive cytotoxic T cells. Gene transfer in DC would be a useful strategy to load DC with relevant de novo synthesized antigens for immunotherapeutical purposes. As a first step towards a DC-based gene therapy, we examined the efficiency of nonviral transfection in different types of cultured human dendritic cells with a humanized red-shifted green fluorescent protein reporter gene. Plasmid DNA transfection by electroporation or lipofection was used to transfect CD34+ progenitor cell-derived DC (PC-DC) and Langerhans' cells (PC-LC), as well as monocyte-derived DC (Mo-DC). While lipofection was unsuccessful in all types of DC, we obtained high-efficiency gene transfer by electroporation in PC-LC (16%) and PC-DC (12%). In contrast, electroporation was strikingly less efficient in Mo-DC (≤ 2%). The potent allostimulatory capacity of DC was still retained in electroporated PC-DC and PC-LC. In conclusion, electroporation of antigen expressing plasmid DNA is an efficient tool for nonviral gene transfer in PC-DC and PC-LC, but not in Mo-DC and could be useful for the development of DC-based tumor immunotherapy.

KW - Dendritic cells

KW - Electroporation

KW - Flow cytometry

KW - Gene transfer

KW - Green fluorescent protein

KW - Transfection efficiency

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DO - 10.1038/sj.gt.3300626

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AN - SCOPUS:7144250527

SN - 0969-7128

VL - 5

SP - 700

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JO - Gene Therapy

JF - Gene Therapy

IS - 5

ER -

Nonviral transfection of distinct types of human dendritic cells: High-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells

Abstract

Keywords

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