Noncontact in vivo scanning laser microscopy of filtering blebs

Zaher Sbeity, Pat Michael Palmiero, Celso Tello, Jeffrey M. Liebmann, Robert Ritch

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Purpose: To evaluate the ability of a noncontact, prototype scanning laser confocal microscope to image microstructural features of filtering blebs in vivo and to correlate these with the clinical features. Methods: Thirty-one blebs of 24 patients underwent noncontact in vivo confocal microscopy using the Rostock Cornea Module of the Heidelberg Retina Tomograph II (50× noncontact Nikon lens, 1-2μm resolution, field of view: 500×500μm). Blebs were clinically classified as successful (low, diffuse, microcystic, and hypovascular) (n=22, mean intraocular pressure<20?mm Hg without hypotensive medications) or failed (flat, cicatrized, and nonfiltering) (n=9, mean intraocular pressure>20mmHg with or without hypotensive medications). Multiple transverse images were taken over the scleral flap at 1 to 2mm from the limbus. Images were evaluated by 2 observers masked to clinical appearance and function of the bleb. Results: Transverse images of the successful blebs showed epithelial microcysts (50 to 100μm) and small-sized to medium-sized (100 to 150μm) subconjunctival cystic spaces with scattered loose collagen-like networks around the scleral flap. Failed blebs showed diffuse hyperreflective dense stroma, increased and tortuous vascularization, and no cystic spaces. The microscopic findings of all blebs were consistent with previous reports of contact in vivo confocal microscopy. Conclusions: Noncontact in vivo confocal microscopy of filtering blebs correlated with the clinical morphology. This noncontact approach provides information about bleb morphology without the risk of bleb microinjury, leak, or infection. Further study and optimization for imaging surface tissues are needed to improve our understanding of bleb maturation and wound healing.

Original languageEnglish
Pages (from-to)479-483
Number of pages5
JournalJournal of Glaucoma
Volume18
Issue number6
DOIs
StatePublished - Aug 2009
Externally publishedYes

Keywords

  • Blebs
  • Confocal laser scanning microscopy
  • Noncontact lens

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