TY - JOUR
T1 - Non-poisson analysis of endonucleases with substrate sequence specificities
AU - Clark Lambert, W.
AU - Lambert, Muriel W.
N1 - Funding Information:
Supported by grants from the Amyotrophic Lateral Sclerosis Association, the March of Dimes Birth Defects Foundation; and the New Jersey Commission on Cancer Research.
PY - 1986
Y1 - 1986
N2 - Endonucleases with substrate-sequence specificities, such as restriction enzymes, usually cleave small, defined nucleic acid molecules used in enzyme assays at one or only a few sites. The methods in common use for analysis of endonucleases are based on the Poisson distribution. A critical, but usually unstated, assumption of this distribution, however, is that there is a large number of possible reactive sites on each substrate molecule. Thus use of the Poisson distribution may introduce large errors into analysis of such assays. Here we develop a series of appropriate expressions for use in analyzing endonucleases with substrate-sequence specificities.
AB - Endonucleases with substrate-sequence specificities, such as restriction enzymes, usually cleave small, defined nucleic acid molecules used in enzyme assays at one or only a few sites. The methods in common use for analysis of endonucleases are based on the Poisson distribution. A critical, but usually unstated, assumption of this distribution, however, is that there is a large number of possible reactive sites on each substrate molecule. Thus use of the Poisson distribution may introduce large errors into analysis of such assays. Here we develop a series of appropriate expressions for use in analyzing endonucleases with substrate-sequence specificities.
UR - http://www.scopus.com/inward/record.url?scp=0022479315&partnerID=8YFLogxK
U2 - 10.1016/0735-0651(86)90003-8
DO - 10.1016/0735-0651(86)90003-8
M3 - Article
AN - SCOPUS:0022479315
SN - 0735-0651
VL - 3
SP - 75
EP - 77
JO - Gene Analysis Techniques
JF - Gene Analysis Techniques
IS - 4
ER -