Non-permissive SARS-CoV-2 infection in human neurospheres

Carolina da S.G. Pedrosa; Livia Goto-Silva; Jairo R. Temerozo; Leticia R.Q. Souza; Gabriela Vitória; Isis M. Ornelas; Karina Karmirian; Mayara A. Mendes; Ismael C. Gomes; Carolina Q. Sacramento; Natalia Fintelman-Rodrigues; Vinicius Cardoso Soares; Suelen da Silva Gomes Dias; José A. Salerno; Teresa Puig-Pijuan; Julia T. Oliveira; Luiz G.H.S. Aragão; Thayana C.Q. Torquato; Carla Veríssimo; Diogo Biagi; Estela M. Cruvinel; Rafael Dariolli; Daniel R. Furtado; Helena L. Borges; Patrícia T. Bozza; Stevens Rehen; Thiago Moreno L. Souza; Marília Zaluar P. Guimarães

doi:10.1016/j.scr.2021.102436

Non-permissive SARS-CoV-2 infection in human neurospheres

Carolina da S.G. Pedrosa
, Livia Goto-Silva
, Jairo R. Temerozo
, Leticia R.Q. Souza
, Gabriela Vitória
, Isis M. Ornelas
, Karina Karmirian
, Mayara A. Mendes
, Ismael C. Gomes
, Carolina Q. Sacramento
, Natalia Fintelman-Rodrigues
, Vinicius Cardoso Soares
, Suelen da Silva Gomes Dias
, José A. Salerno
, Teresa Puig-Pijuan
, Julia T. Oliveira
, Luiz G.H.S. Aragão
, Thayana C.Q. Torquato
, Carla Veríssimo
, Diogo Biagi

Estela M. Cruvinel, Rafael Dariolli, Daniel R. Furtado, Helena L. Borges, Patrícia T. Bozza, Stevens Rehen, Thiago Moreno L. Souza, Marília Zaluar P. Guimarães

Icahn School of Medicine at Mount Sinai

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including the brain. Neurological manifestations such as stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but the neurotropic potential of the virus is still debated. Herein, we sought to investigate SARS-CoV-2 infection in human neural cells. We demonstrated that SARS-CoV-2 infection of neural tissue is non-permissive, however, it can elicit inflammatory response and cell damage. These findings add to the hypothesis that most of the neural damage caused by SARS-CoV-2 infection is due to a systemic inflammation leading to indirect harmful effects on the central nervous system despite the absence of local viral replication.

Original language	English
Article number	102436
Journal	Stem Cell Research
Volume	54
DOIs	https://doi.org/10.1016/j.scr.2021.102436
State	Published - Jul 2021

Keywords

Brain
Neurospheres
New coronavirus
SARS-CoV-2
iPS

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Pedrosa, C. D. S. G., Goto-Silva, L., Temerozo, J. R., Souza, L. R. Q., Vitória, G., Ornelas, I. M., Karmirian, K., Mendes, M. A., Gomes, I. C., Sacramento, C. Q., Fintelman-Rodrigues, N., Cardoso Soares, V., Silva Gomes Dias, S. D., Salerno, J. A., Puig-Pijuan, T., Oliveira, J. T., Aragão, L. G. H. S., Torquato, T. C. Q., Veríssimo, C., ... Guimarães, M. Z. P. (2021). Non-permissive SARS-CoV-2 infection in human neurospheres. Stem Cell Research, 54, Article 102436. https://doi.org/10.1016/j.scr.2021.102436

@article{d2246eab04ab4ea7af04f9f3d5a96ab4,

title = "Non-permissive SARS-CoV-2 infection in human neurospheres",

abstract = "Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including the brain. Neurological manifestations such as stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but the neurotropic potential of the virus is still debated. Herein, we sought to investigate SARS-CoV-2 infection in human neural cells. We demonstrated that SARS-CoV-2 infection of neural tissue is non-permissive, however, it can elicit inflammatory response and cell damage. These findings add to the hypothesis that most of the neural damage caused by SARS-CoV-2 infection is due to a systemic inflammation leading to indirect harmful effects on the central nervous system despite the absence of local viral replication.",

keywords = "Brain, Neurospheres, New coronavirus, SARS-CoV-2, iPS",

author = "Pedrosa, \{Carolina da S.G.\} and Livia Goto-Silva and Temerozo, \{Jairo R.\} and Souza, \{Leticia R.Q.\} and Gabriela Vit{\'o}ria and Ornelas, \{Isis M.\} and Karina Karmirian and Mendes, \{Mayara A.\} and Gomes, \{Ismael C.\} and Sacramento, \{Carolina Q.\} and Natalia Fintelman-Rodrigues and \{Cardoso Soares\}, Vinicius and \{Silva Gomes Dias\}, \{Suelen da\} and Salerno, \{Jos{\'e} A.\} and Teresa Puig-Pijuan and Oliveira, \{Julia T.\} and Arag{\~a}o, \{Luiz G.H.S.\} and Torquato, \{Thayana C.Q.\} and Carla Ver{\'i}ssimo and Diogo Biagi and Cruvinel, \{Estela M.\} and Rafael Dariolli and Furtado, \{Daniel R.\} and Borges, \{Helena L.\} and Bozza, \{Patr{\'i}cia T.\} and Stevens Rehen and \{Moreno L. Souza\}, Thiago and Guimar{\~a}es, \{Mar{\'i}lia Zaluar P.\}",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = jul,

doi = "10.1016/j.scr.2021.102436",

language = "English",

volume = "54",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

}

Pedrosa, CDSG, Goto-Silva, L, Temerozo, JR, Souza, LRQ, Vitória, G, Ornelas, IM, Karmirian, K, Mendes, MA, Gomes, IC, Sacramento, CQ, Fintelman-Rodrigues, N, Cardoso Soares, V, Silva Gomes Dias, SD, Salerno, JA, Puig-Pijuan, T, Oliveira, JT, Aragão, LGHS, Torquato, TCQ, Veríssimo, C, Biagi, D, Cruvinel, EM, Dariolli, R, Furtado, DR, Borges, HL, Bozza, PT, Rehen, S, Moreno L. Souza, T & Guimarães, MZP 2021, 'Non-permissive SARS-CoV-2 infection in human neurospheres', Stem Cell Research, vol. 54, 102436. https://doi.org/10.1016/j.scr.2021.102436

TY - JOUR

T1 - Non-permissive SARS-CoV-2 infection in human neurospheres

AU - Pedrosa, Carolina da S.G.

AU - Goto-Silva, Livia

AU - Temerozo, Jairo R.

AU - Souza, Leticia R.Q.

AU - Vitória, Gabriela

AU - Ornelas, Isis M.

AU - Karmirian, Karina

AU - Mendes, Mayara A.

AU - Gomes, Ismael C.

AU - Sacramento, Carolina Q.

AU - Fintelman-Rodrigues, Natalia

AU - Cardoso Soares, Vinicius

AU - Silva Gomes Dias, Suelen da

AU - Salerno, José A.

AU - Puig-Pijuan, Teresa

AU - Oliveira, Julia T.

AU - Aragão, Luiz G.H.S.

AU - Torquato, Thayana C.Q.

AU - Veríssimo, Carla

AU - Biagi, Diogo

AU - Cruvinel, Estela M.

AU - Dariolli, Rafael

AU - Furtado, Daniel R.

AU - Borges, Helena L.

AU - Bozza, Patrícia T.

AU - Rehen, Stevens

AU - Moreno L. Souza, Thiago

AU - Guimarães, Marília Zaluar P.

PY - 2021/7

Y1 - 2021/7

N2 - Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including the brain. Neurological manifestations such as stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but the neurotropic potential of the virus is still debated. Herein, we sought to investigate SARS-CoV-2 infection in human neural cells. We demonstrated that SARS-CoV-2 infection of neural tissue is non-permissive, however, it can elicit inflammatory response and cell damage. These findings add to the hypothesis that most of the neural damage caused by SARS-CoV-2 infection is due to a systemic inflammation leading to indirect harmful effects on the central nervous system despite the absence of local viral replication.

AB - Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including the brain. Neurological manifestations such as stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but the neurotropic potential of the virus is still debated. Herein, we sought to investigate SARS-CoV-2 infection in human neural cells. We demonstrated that SARS-CoV-2 infection of neural tissue is non-permissive, however, it can elicit inflammatory response and cell damage. These findings add to the hypothesis that most of the neural damage caused by SARS-CoV-2 infection is due to a systemic inflammation leading to indirect harmful effects on the central nervous system despite the absence of local viral replication.

KW - Brain

KW - Neurospheres

KW - New coronavirus

KW - SARS-CoV-2

KW - iPS

UR - https://www.scopus.com/pages/publications/85111042491

U2 - 10.1016/j.scr.2021.102436

DO - 10.1016/j.scr.2021.102436

M3 - Article

C2 - 34186311

AN - SCOPUS:85111042491

SN - 1873-5061

VL - 54

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102436

ER -

Non-permissive SARS-CoV-2 infection in human neurospheres

Abstract

Keywords

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