N,N,N-Trimethyl chitosan nanoparticles for the delivery of monoclonal antibodies against hepatocellular carcinoma cells

Preeyanat Vongchan, Yupanan Wutti-In, Warayuth Sajomsang, Pattarapond Gonil, Suchart Kothan, Robert J. Linhardt

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

N,N,N-Trimethyl chitosan chloride is capable of forming nanocomplexes with protein through ionotropic gelation. A monoclonal antibody, raised against human liver heparan sulfate proteoglycan and specifically inhibiting hepatocellular carcinoma in vitro, was prepared in nanocomplexes of this modified chitosan. The smallest nanocomplexes (59 ± 17 nm, zeta-potential 16.5 ± 0.5 mV) were obtained at polysaccharide:antibody ratios of 5:0.3. Spherical particles with a smooth surface and compact structure having a mean diameter of ∼11.2 ± 0.09 nm were investigated by atomic force microscopy. Cellular uptake of fluorescently labeled nanocomplexes was studied in mouse monocyte models of cancer and normal cells. External and internal fluorescence was analyzed by flow cytometry. The results demonstrate that the nanocomplexes could enter cells and were retained for a longer period of time in cancer cells where they exhibited greater toxicity. These nanocomplexes appear safe and could potentially enhance the half-life of added antibodies.

Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalCarbohydrate Polymers
Volume85
Issue number1
DOIs
StatePublished - 22 Apr 2011
Externally publishedYes

Keywords

  • Chitosan
  • Heparan sulfate proteoglycans
  • Hepatocellular carcinoma
  • Monoclonal antibody
  • N,N,N-Trimethyl chitosan nanocomplexes

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