NME proteins regulate class switch recombination

Simin Zheng, Anthony Kusnadi, Jee Eun Choi, Bao Q. Vuong, Daniela Rhodes, Jayanta Chaudhuri

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Class switch recombination (CSR) in B cells involves deletion-recombination at switch (S) region DNA and is important for the diversification of antibody isotypes during an immune response. Here, we identify two NME [NM23/NDPK (nucleoside diphosphate kinase)] isoforms, NME1 and NME2, as novel players in this process. Knockdown of NME2 leads to decreased CSR, while knockdown of the highly homologous NME1 results in increased CSR. Interestingly, these NME proteins also display differential occupancy at S regions during CSR despite their homology; NME1 binds to S regions prior to stimulation, while NME2 binds to S regions only after stimulation. To the best of our knowledge, this represents the first report of a role for these proteins in the regulation of CSR.

Original languageEnglish
Pages (from-to)80-87
Number of pages8
JournalFEBS Letters
Volume593
Issue number1
DOIs
StatePublished - Jan 2019
Externally publishedYes

Keywords

  • DNA recombination
  • G-quadruplex
  • protein–DNA interaction

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