TY - JOUR
T1 - Nipah virus edits its P gene at high frequency to express the V and W proteins
AU - Kulkarni, Sachin
AU - Volchkova, Valentina
AU - Basler, Christopher F.
AU - Palese, Peter
AU - Volchkov, Viktor E.
AU - Shaw, Megan L.
PY - 2009/4
Y1 - 2009/4
N2 - Nipah virus (NiV) is predicted to encode four proteins from its P gene (P, V, W, and C) via mRNA editing and an alternate open reading frame. By use of specific antibodies, the expression of the V, W, and C proteins in NiV-infected cells has now been confirmed. Analysis of the P-gene transcripts shows a ratio of P:V:W mRNA of 1:1:1, but this differs over time, with greater proportions of V and W transcripts observed as the infection progresses. Eighty-two percent of transcripts are edited, with up to 11 G insertions observed. This exceptionally high editing frequency ensures expression of the V and W proteins.
AB - Nipah virus (NiV) is predicted to encode four proteins from its P gene (P, V, W, and C) via mRNA editing and an alternate open reading frame. By use of specific antibodies, the expression of the V, W, and C proteins in NiV-infected cells has now been confirmed. Analysis of the P-gene transcripts shows a ratio of P:V:W mRNA of 1:1:1, but this differs over time, with greater proportions of V and W transcripts observed as the infection progresses. Eighty-two percent of transcripts are edited, with up to 11 G insertions observed. This exceptionally high editing frequency ensures expression of the V and W proteins.
UR - http://www.scopus.com/inward/record.url?scp=64049083722&partnerID=8YFLogxK
U2 - 10.1128/JVI.02599-08
DO - 10.1128/JVI.02599-08
M3 - Article
C2 - 19211754
AN - SCOPUS:64049083722
SN - 0022-538X
VL - 83
SP - 3982
EP - 3987
JO - Journal of Virology
JF - Journal of Virology
IS - 8
ER -