Nipah Virus Bangladesh Infection Elicits Organ-Specific Innate and Inflammatory Responses in the Marmoset Model

Christian S. Stevens, Jake Lowry, Terry Juelich, Colm Atkins, Kendra Johnson, Jennifer K. Smith, Maryline Panis, Tetsuro Ikegami, Benjamin Tenoever, Alexander N. Freiberg, Benhur Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The common marmoset (Callithrix jacchus) is increasingly recognized as an ideal nonhuman primate (NHP) at high biocontainment due to its smaller size and relative ease of handling. Here, we evaluated the susceptibility and pathogenesis of Nipah virus Bangladesh strain (NiVB) infection in marmosets at biosafety level 4. Infection via the intranasal and intratracheal route resulted in fatal disease in all 4 infected marmosets. Three developed pulmonary edema and hemorrhage as well as multifocal hemorrhagic lymphadenopathy, while 1 recapitulated neurologic clinical manifestations and cardiomyopathy on gross pathology. Organ-specific innate and inflammatory responses were characterized by RNA sequencing in 6 different tissues from infected and control marmosets. Notably, a unique transcriptome was revealed in the brainstem of the marmoset exhibiting neurological signs. Our results provide a more comprehensive understanding of NiV pathogenesis in an accessible and novel NHP model, closely reflecting clinical disease as observed in NiV patients.

Original languageEnglish
Pages (from-to)604-614
Number of pages11
JournalJournal of Infectious Diseases
Volume228
Issue number5
DOIs
StatePublished - 1 Sep 2023

Keywords

  • Nipah virus Bangladesh
  • RNA-seq
  • cardiomyopathy
  • common marmoset
  • inflammatory response
  • neurological disease
  • nonhuman primate
  • pathogenesis
  • respiratory disease

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