Nifedipine blocks calcium-dependent cholinergic depolarization in the guinea pig hippocampus

Robert D. Blitzer, Orlando Gil, Gila Omri, Emmanuel M. Landau

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The possibility that cholinergic stimulation might directly activate a receptor-operated Ca2+ channel was investigated in the CA1 region of guinea pig hippocampus using intracellular recording techniques. Two cholinergic responses were studied: (1) the plateau depolarization evoked by cholinergic stimulation in the presence of Ba2+; and (2) the Ca2+-dependent component of membrane depolarization. Both of these responses were blocked by 1-5 μM of nifedipine, a blocker of voltage-dependent L-type Ca2+ channels. In addition, the plateau response was mimicked by direct postsynaptic depolarization in the presence of Ba2+. We conclude that cholinergic stimulation does not directly activate a Ca2+ conductance in these neurons, but rather leads to the indirect activation of L channels which may be located both pre- and postsynaptically.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalBrain Research
Volume542
Issue number2
DOIs
StatePublished - 1 Mar 1991

Keywords

  • Acetylcholine
  • Barium
  • Calcium
  • Guinea pig
  • Hippocampus
  • Nifedipine

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