Niemann-pick C1 is a late endosome-resident protein that transiently associates with lysosomes and the trans-Golgi network

Maureen E. Higgins, Joanna P. Davies, Fannie W. Chen, Yiannis A. Ioannou

Research output: Contribution to journalArticlepeer-review

207 Scopus citations

Abstract

Niemann-Pick type C (NPC) disease is a severe cell lipidosis characterized by the accumulation of unesterified cholesterol in the endosomal/lysosomal system. Recently the primary disease-causing gene, NPC1, was identified, but few clues regarding its potential function(s) could be derived from its predicted amino acid sequence. Therefore, efforts were directed at characterizing the subcellular location of the NPC1 protein. Initial studies with a FLAG-tagged NPC1 cDNA demonstrated that NPC1 is a glycoprotein that associates with the membranes of a population of cytoplasmic vesicles. Immunofluorescence microscopy using anti-NPC1 polyclonal antibodies confirmed this analysis. Double-label immunofluorescence microscopy and subcellular fractionation studies indicated that NPC1 associates predominantly with late endosomes (Rab9 GTPase-positive vesicles) and, to a lesser extent, with lysosomes and the trans-Golgi network. When cholesterol egress from lysosomes was blocked by treatment of cells with U18666A, the NPC1 location shifted from late endosomes to the trans-Golgi network and lysosomes. Subcellular fractionation of liver homogenates from U18666A-treated mice confirmed these observations. These data suggest that U18666A may inhibit the retrograde transport of NPC1 from lysosomes to late endosomes for subsequent transfer to the trans-Golgi network.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalMolecular Genetics and Metabolism
Volume68
Issue number1
DOIs
StatePublished - Sep 1999

Keywords

  • Cholesterol transport
  • Endosomes
  • Low-density lipoprotein-derived cholesterol
  • Niemann-Pick C1
  • U18666A

Fingerprint

Dive into the research topics of 'Niemann-pick C1 is a late endosome-resident protein that transiently associates with lysosomes and the trans-Golgi network'. Together they form a unique fingerprint.

Cite this