Next generation sequencing of the pig αβ TCR repertoire identifies the porcine invariant NKT cell receptor

Guan Yang, Bianca L. Artiaga, Carrie L. Lomelino, Anitha D. Jayaprakash, Ravi Sachidanandam, Robert McKenna, John P. Driver

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Swine represent the only livestock with an estαβlished invariant NKT (iNKT) cell-CD1d system. In this study, we exploited the fact that pig iNKT cells can be purified using a mouse CD1d tetramer reagent to estαβlish their TCR repertoire by next generation sequencing. CD1d tetramer-positive pig cells predominantly expressed an invariant Vα-Jα rearrangement, without nontemplate nucleotide diversity, homologous to the Vα24-Jα18 and Vα14-Jα18 rearrangements of human and murine iNKT cells. The coexpressed β-chain used a Vβ segment homologous to the semivariant Vβ11 and Vβ8.2 segments of human and murine iNKT cell receptors. Molecular modeling found that contacts within CD1d and CDR1a that underlie fine specificity differences between mouse and human iNKT cells are conserved between pigs and humans, indicating that the response of porcine and human iNKT cells to CD1d-restricted Ags may be similar. Accordingly, pigs, which are an important species for diverse fields of biomedical research, may be useful for developing human-based iNKT cell therapies for cancer, infectious diseases, and other disorders. Our study also sequenced the expressed TCR repertoire of conventional porcine αβ T cells, which identified 48 Vα, 50 Jα, 18 Vβ, and 18 Jβ sequences, most of which correspond to human gene segments. These findings provide information on the αβ TCR usage of pigs, which is understudied and deserves further attention.

Original languageEnglish
Pages (from-to)1981-1991
Number of pages11
JournalJournal of Immunology
Volume202
Issue number7
DOIs
StatePublished - 2019

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