New Metabolites of 11β-Hydroxy-Δ4-androstene-3,17-dione

David K. Fukushima; Shirley Dobriner; H. Leon Bradlow; Barnett Zumoff; Leon Hellman; T. F. Gallagher

doi:10.1021/bi00870a001

New Metabolites of 11β-Hydroxy-Δ4-androstene-3,17-dione

David K. Fukushima, Shirley Dobriner, H. Leon Bradlow, Barnett Zumoff, Leon Hellman, T. F. Gallagher

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

3β, 11 β-Dihydroxy-Δ4-androsten-17-one has been identified as a metabolite of 11β-hydroxy-Δ4-androstene-3,17-dione in man. Its epimer, 3β,11β-dihydroxy-Δ4-androsten-17-one, was also isolated from urine but it was shown to be derived from the 3Δ-hydroxy compound during the mild acid conditions of hydrolysis. The unsaturated metabolite was excreted in conjugated form which could be cleaved by treatment at pH 5.0 at 45° for 1 day. Longer periods of incubation resulted in a 1:1 equilibrium mixture of the two epimers.

Original language	English
Pages (from-to)	1779-1783
Number of pages	5
Journal	Biochemistry
Volume	5
Issue number	6
DOIs	https://doi.org/10.1021/bi00870a001
State	Published - 1966
Externally published	Yes

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title = "New Metabolites of 11β-Hydroxy-Δ4-androstene-3,17-dione",

abstract = "3β, 11 β-Dihydroxy-Δ4-androsten-17-one has been identified as a metabolite of 11β-hydroxy-Δ4-androstene-3,17-dione in man. Its epimer, 3β,11β-dihydroxy-Δ4-androsten-17-one, was also isolated from urine but it was shown to be derived from the 3Δ-hydroxy compound during the mild acid conditions of hydrolysis. The unsaturated metabolite was excreted in conjugated form which could be cleaved by treatment at pH 5.0 at 45° for 1 day. Longer periods of incubation resulted in a 1:1 equilibrium mixture of the two epimers.",

author = "Fukushima, {David K.} and Shirley Dobriner and Bradlow, {H. Leon} and Barnett Zumoff and Leon Hellman and Gallagher, {T. F.}",

year = "1966",

doi = "10.1021/bi00870a001",

language = "English",

volume = "5",

pages = "1779--1783",

journal = "Biochemistry",

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publisher = "American Chemical Society",

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T1 - New Metabolites of 11β-Hydroxy-Δ4-androstene-3,17-dione

AU - Fukushima, David K.

AU - Dobriner, Shirley

AU - Bradlow, H. Leon

AU - Zumoff, Barnett

AU - Hellman, Leon

AU - Gallagher, T. F.

PY - 1966

Y1 - 1966

N2 - 3β, 11 β-Dihydroxy-Δ4-androsten-17-one has been identified as a metabolite of 11β-hydroxy-Δ4-androstene-3,17-dione in man. Its epimer, 3β,11β-dihydroxy-Δ4-androsten-17-one, was also isolated from urine but it was shown to be derived from the 3Δ-hydroxy compound during the mild acid conditions of hydrolysis. The unsaturated metabolite was excreted in conjugated form which could be cleaved by treatment at pH 5.0 at 45° for 1 day. Longer periods of incubation resulted in a 1:1 equilibrium mixture of the two epimers.

AB - 3β, 11 β-Dihydroxy-Δ4-androsten-17-one has been identified as a metabolite of 11β-hydroxy-Δ4-androstene-3,17-dione in man. Its epimer, 3β,11β-dihydroxy-Δ4-androsten-17-one, was also isolated from urine but it was shown to be derived from the 3Δ-hydroxy compound during the mild acid conditions of hydrolysis. The unsaturated metabolite was excreted in conjugated form which could be cleaved by treatment at pH 5.0 at 45° for 1 day. Longer periods of incubation resulted in a 1:1 equilibrium mixture of the two epimers.

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AN - SCOPUS:0013914419

SN - 0006-2960

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SP - 1779

EP - 1783

JO - Biochemistry

JF - Biochemistry

IS - 6

ER -

New Metabolites of 11β-Hydroxy-Δ4-androstene-3,17-dione

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