New insights on the pbmcs phospholipidome in obesity demonstrate modulations associated with insulin resistance and glycemic status

Chloé Wilkin, Megan Colonval, Jonas Dehairs, Nathalie Esser, Margaud Iovino, Marco A. Gianfrancesco, Marjorie Fadeur, Johan V. Swinnen, Nicolas Paquot, Jacques Piette, Sylvie Legrand-Poels

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

(1) Background: Obesity and type 2 diabetes have been suspected to impact both intrin-sic metabolism and function of circulating immune cells. (2) Methods: To further investigate this immunometabolic modulation, we profiled the phospholipidome of the peripheral blood mononu-clear cells (PBMCs) in lean, normoglycemic obese (OBNG) and obese with dysglycemia (OBDysG) individuals. (3) Results: The global PBMCs phospholipidome is significantly downmodulated in OBDysG unlike OBNG patients when compared to lean ones. Multiple linear regression analyses show a strong negative relationship between the global PBMCs phospholipidome and parameters assessing insulin resistance. Even though all classes of phospholipid are affected, the relative abun-dance of each class is maintained with the exception of Lyso-PC/PC and Lyso-PE/PE ratios that are downmodulated in PBMCs of OBDysG compared to OBNG individuals. Interestingly, the percentage of saturated PC is positively associated with glycated hemoglobin (HbA1c). Moreover, a few lipid species are significantly downmodulated in PBMCs of OBDysG compared to OBNG individuals, making possible to distinguish the two phenotypes. (4) Conclusions: This lipidomic study highlights for the first-time modulations of the PBMCs phospholipidome in obese patients with prediabetes and type 2 diabetes. Such phospholipidome remodeling could disrupt the cell membranes and the lipid mediator’s levels, driving an immune cell dysfunction.

Original languageEnglish
Article number3461
JournalNutrients
Volume13
Issue number10
DOIs
StatePublished - Oct 2021
Externally publishedYes

Keywords

  • Immunology
  • Lipidomic
  • Membrane lipids
  • Metabolism
  • Obesity
  • Phospholipids
  • Type 2 diabetes

Fingerprint

Dive into the research topics of 'New insights on the pbmcs phospholipidome in obesity demonstrate modulations associated with insulin resistance and glycemic status'. Together they form a unique fingerprint.

Cite this