Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary

Lloyd D. Fricker; Alexandre K. Tashima; Amanda K. Fakira; Ute Hochgeschwender; William C. Wetsel; Lakshmi A. Devi

doi:10.1016/j.chembiol.2020.11.003

Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary

Lloyd D. Fricker
, Alexandre K. Tashima
, Amanda K. Fakira
, Ute Hochgeschwender
, William C. Wetsel
, Lakshmi A. Devi

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We report a method to identify neuropeptides in specific cell types, using mice with a conditional deletion of carboxypeptidase E. It was validated by deleting CPE in proopiomelanocortin cells. Surprisingly, these mice have greatly reduced levels of the anorexigenic peptide α-MSH but are not obese.

Original language	English
Pages (from-to)	105-112.e4
Journal	Cell Chemical Biology
Volume	28
Issue number	1
DOIs	https://doi.org/10.1016/j.chembiol.2020.11.003
State	Published - 21 Jan 2021

Keywords

Proteomics
carboxypeptidase
neuropeptide
neuropeptidomics
peptidomics

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10.1016/j.chembiol.2020.11.003

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title = "Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary",

abstract = "We report a method to identify neuropeptides in specific cell types, using mice with a conditional deletion of carboxypeptidase E. It was validated by deleting CPE in proopiomelanocortin cells. Surprisingly, these mice have greatly reduced levels of the anorexigenic peptide α-MSH but are not obese.",

keywords = "Proteomics, carboxypeptidase, neuropeptide, neuropeptidomics, peptidomics",

author = "Fricker, \{Lloyd D.\} and Tashima, \{Alexandre K.\} and Fakira, \{Amanda K.\} and Ute Hochgeschwender and Wetsel, \{William C.\} and Devi, \{Lakshmi A.\}",

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AU - Fricker, Lloyd D.

AU - Tashima, Alexandre K.

AU - Fakira, Amanda K.

AU - Hochgeschwender, Ute

AU - Wetsel, William C.

AU - Devi, Lakshmi A.

PY - 2021/1/21

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AB - We report a method to identify neuropeptides in specific cell types, using mice with a conditional deletion of carboxypeptidase E. It was validated by deleting CPE in proopiomelanocortin cells. Surprisingly, these mice have greatly reduced levels of the anorexigenic peptide α-MSH but are not obese.

KW - Proteomics

KW - carboxypeptidase

KW - neuropeptide

KW - neuropeptidomics

KW - peptidomics

UR - https://www.scopus.com/pages/publications/85097213410

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DO - 10.1016/j.chembiol.2020.11.003

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SP - 105-112.e4

JO - Cell Chemical Biology

JF - Cell Chemical Biology

IS - 1

ER -

Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary

Abstract

Keywords

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