Neuropeptidergic inhibitory regulation of Met-enkephalin immunoreactive material release from rat spinal cord in vitro

U. Uzumaki, S. Govoni, E. Faccini, G. Pasinetti, C. Missale, M. Trabucchi

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The release of Met-enkephalin immunoreactive material (ME-IR) from rat spinal slices was measured in vitro. This release increased about 4 fold in response to the addition of K+ ions. K+-evoked release of ME-IR was Ca++ dependent. Veratridine, a depolarizing agent, also stimulated the release of ME-IR. Veratridine-induced ME-IR release was completely prevented by tetrodotoxin (TTX), a Na+ channel blocker. Somatostatin (SRIF) inhibited both basal and K+-evoked release of ME-IR at 10-7 M. Substance P had a similar effect although higher concentrations were needed. γ-Aminobutyric acid (GABA) and neurotensin (NT) did not affect the basal release but slightly decreased K+-evoked release at 10-5 M. Serotonin (5-HT) and noradrenaline (NA), did not affect ME-IR release. These results suggest that some of the neuropeptides present in the spinal cord, especially SP and SRIF, may be potent modulators of ME-IR release at the spinal level.

Original languageEnglish
Pages (from-to)849-852
Number of pages4
JournalPeptides
Volume5
Issue number5
DOIs
StatePublished - 1984
Externally publishedYes

Keywords

  • Met-enkephalin release
  • Somatostatin
  • Spinal cord
  • Substance P

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