Nerve and epidermal growth factors induce the release of the Alzheimer Amyloid precursor from PC 12 cell cultures

Lawrence M. Refolo, Steven R.J. Salton, John P. Anderson, Pankaj Mehta, Nikolaos K. Robakis

Research output: Contribution to journalArticlepeer-review

113 Scopus citations

Abstract

Antisera against specific sites of the Alzheimer βAmyloid protein precursor (βAPP) were used to study the effects of nerve and epidermal growth factors on the expression and processing of this protein in PC12 cell cultures. Two major βAPP proteins (140 and 105 kDa) both containing the Kunitz-protease inhibitor insert (KPI), were detected in cell extracts of naive PC12 cells. Treatment of these cultures with nerve growth factor (NGF) induced the release of two βAPP species 125 and 120 kDa, both of which contained the KPI domain and lacked the carboxy-terminal portion of the precursor. The released βAPP contained O-linked sugars. Onlh one of the released βAPP proteins bound to the lectin Concanavalin A indicating that they differ in their glycosylation. Epidermal growth factor (EGF) also induced the release of βAPP proteins into the culture medium with similar electrophoretic mobilities as those released by NGF.

Original languageEnglish
Pages (from-to)664-670
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume164
Issue number2
DOIs
StatePublished - 31 Oct 1989

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