Near-Infrared Intraoperative Chemiluminescence Imaging

Gabriel E. Büchel, Brandon Carney, Travis M. Shaffer, Jun Tang, Christine Austin, Manish Arora, Brian M. Zeglis, Jan Grimm, Jörg Eppinger, Thomas Reiner

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Intraoperative imaging technologies recently entered the operating room, and their implementation is revolutionizing how physicians plan, monitor, and perform surgical interventions. In this work, we present a novel surgical imaging reporter system: intraoperative chemiluminescence imaging (ICI). To this end, we have leveraged the ability of a chemiluminescent metal complex to generate near-infrared light upon exposure to an aqueous solution of Ce4+in the presence of reducing tissue or blood components. An optical camera spatially resolves the resulting photon flux. We describe the construction and application of a prototype imaging setup, which achieves a detection limit as low as 6.9 pmol cm−2of the transition-metal-based ICI agent. As a proof of concept, we use ICI for the in vivo detection of our transition metal tracer following both systemic and subdermal injections. The very high signal-to-noise ratios make ICI an interesting candidate for the development of new intraoperative imaging technologies.

Original languageEnglish
Pages (from-to)1978-1982
Number of pages5
JournalChemMedChem
DOIs
StatePublished - 2016

Keywords

  • chemiluminescence
  • intraoperative imaging
  • lymph node imaging
  • near infrared
  • ruthenium

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