TY - JOUR
T1 - nanos1
T2 - A mouse nanos gene expressed in the central nervous system is dispensable for normal development
AU - Haraguchi, Seiki
AU - Tsuda, Masayuki
AU - Kitajima, Satoshi
AU - Sasaoka, Yumiko
AU - Nomura-Kitabayashi, Aya
AU - Kurokawa, Kiyoshi
AU - Saga, Yumiko
N1 - Funding Information:
We thank Mariko Ikumi, Seiko Shinzawa and Maho Endo for general technical assistance and Takefumi Yamamoto for technical assistance in electron microscopy. We also thank Tatsumi Hirata and Fumikazu Suto for valuable discussion. This work was supported by Grants-in-Aid for a Research Project for Future Program from the Japan Society for the Promotion of Sciences and a CREST (Core Research for Evolutional Science and Technology) project from Japan Science and Technology Cooperation, and NIG Cooperative Research Program.
PY - 2003/6
Y1 - 2003/6
N2 - A mouse nanos (nanos1) gene was cloned and its function was examined by generating a gene-knockout mouse. The nanos1 gene encodes an RNA-binding protein, which contains a putative zinc-finger motif that exhibits similarity with other nanos-class genes in vertebrates and invertebrates. Although nanos1 is not detected in primordial germ cells, it is observed in seminiferous tubules of mature testis. Interestingly, maternally expressed nanos1 is observed in substantial amounts in oocytes, but the amount of maternal RNA is rapidly reduced after fertilization, and the transient zygotic nanos1 expression is observed in eight-cell embryos. At 12.5 days postcoitum, nanos1 is re-expressed in the central nervous system and the expression continues in the adult brain, in which the hippocampal formation is the predominant region. The nanos1 -deficient mice develop to term without any detectable abnormality and they are fertile. No significant neural defect is observed in terms of their behavior to date.
AB - A mouse nanos (nanos1) gene was cloned and its function was examined by generating a gene-knockout mouse. The nanos1 gene encodes an RNA-binding protein, which contains a putative zinc-finger motif that exhibits similarity with other nanos-class genes in vertebrates and invertebrates. Although nanos1 is not detected in primordial germ cells, it is observed in seminiferous tubules of mature testis. Interestingly, maternally expressed nanos1 is observed in substantial amounts in oocytes, but the amount of maternal RNA is rapidly reduced after fertilization, and the transient zygotic nanos1 expression is observed in eight-cell embryos. At 12.5 days postcoitum, nanos1 is re-expressed in the central nervous system and the expression continues in the adult brain, in which the hippocampal formation is the predominant region. The nanos1 -deficient mice develop to term without any detectable abnormality and they are fertile. No significant neural defect is observed in terms of their behavior to date.
KW - Germ cell development
KW - Hippocampal formation
KW - Knockout mouse
KW - Nanos
UR - https://www.scopus.com/pages/publications/0038016507
U2 - 10.1016/S0925-4773(03)00043-1
DO - 10.1016/S0925-4773(03)00043-1
M3 - Article
C2 - 12834871
AN - SCOPUS:0038016507
SN - 0925-4773
VL - 120
SP - 721
EP - 731
JO - Mechanisms of Development
JF - Mechanisms of Development
IS - 6
ER -