Nanometer resolution tracking and modeling of bidirectional axonal cargo transport

Minhua Qiu, Hao Chih Lee, Ge Yang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

10 Scopus citations

Abstract

Survival and function of neurons depend critically on bidirectional transport of essential cargoes within their axons. To study related molecular mechanisms, we developed an automated single particle tracking method that combines nanometer resolution detection of cargo positions with kymograph-based multiple hypothesis tracking of cargo motion for precise and complete recovery of cargo trajectories. Experiments confirmed that the method can precisely track vesicles in dense axonal traffic of Drosophila motor neurons at 1020 nm resolution and reliably resolve their trajectories in events of crossing, merging, and splitting. Recovered cargo trajectories were statistically analyzed to identify motion patterns and computationally represented using hidden Markov models to characterize behavior changes. Together, these tools allowed us to reliably detect and measure subtle changes in dynamics of axonal transport and to discover previously unknown axonal transport properties.

Original languageEnglish
Title of host publication2012 9th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI 2012 - Proceedings
Pages992-995
Number of pages4
DOIs
StatePublished - 2012
Externally publishedYes
Event2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Barcelona, Spain
Duration: 2 May 20125 May 2012

Publication series

NameProceedings - International Symposium on Biomedical Imaging
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Conference

Conference2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012
Country/TerritorySpain
CityBarcelona
Period2/05/125/05/12

Keywords

  • axonal transport
  • fluorescence imaging
  • image analysis
  • kymograph
  • single particle tracking

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